Recombinant Schizosaccharomyces pombe DNA damage checkpoint protein rad24 (rad24)

Code CSB-YP337162SXV
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Source Yeast
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Code CSB-EP337162SXV
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Source E.coli
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Code CSB-EP337162SXV-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP337162SXV
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Source Baculovirus
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Code CSB-MP337162SXV
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
rad24
Uniprot No.
Alternative Names
rad24; SPAC8E11.02c; DNA damage checkpoint protein rad24
Species
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Expression Region
1-270
Target Protein Sequence
MSTTSREDAV YLAKLAEQAE RYEGMVENMK SVASTDQELT VEERNLLSVA YKNVIGARRA SWRIVSSIEQ KEESKGNTAQ VELIKEYRQK IEQELDTICQ DILTVLEKHL IPNAASAESK VFYYKMKGDY YRYLAEFAVG EKRQHSADQS LEGYKAASEI ATAELAPTHP IRLGLALNFS VFYYEILNSP DRACYLAKQA FDEAISELDS LSEESYKDST LIMQLLRDNL TLWTSDAEYS AAAAGGNTEG AQENAPSNAP EGEAEPKADA
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Acts in cell cycle and stress checkpoint signaling by sequestering signal transducers regulated by the checkpoints. Required for the DNA damage checkpoint that ensures that DNA damage is repaired before mitosis is attempted. During environmental stress, sequesters srk1-phosphorylated cdc25 in the cytoplasm to delay the G2/M transition. Sequesters byr2 in the cytoplasm to prevent its translocation to the plasma membrane. Sequesters ran1/pat1-phosphorylated mei2 from its non-coding RNA activators (including meiRNA), to prevent meiotic induction in vegetative cells and to regulate meiosis I.
Gene References into Functions
  1. The loss of rad24 in ssp1(-) cells reduces the severity of hyperosmotic stress response and relieves mitotic delay. PMID: 24451546
  2. Rad24p-mediated cytoplasmic retention of Clp1p/Flp1p is important for cell viability upon stress to the cell division apparatus. PMID: 16085489
  3. data presented here show that two elements of fission yeast DNA structure checkpoints, Rad26 and Rad24, define a pathway that responds to microtubule destabilizing conditions PMID: 16930478
  4. The 14-3-3 protein Rad24p is involved in negative control of pho1 gene expression in Schizosaccharomyces pombe. PMID: 17173334
  5. Identification of sam4 as an allele of rad24. PMID: 19584544

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Subcellular Location
Cytoplasm.
Protein Families
14-3-3 family
Database Links
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