Recombinant Schizosaccharomyces pombe G2/mitotic-specific cyclin cig2 (cig2)

Code CSB-YP339711SXV
MSDS
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Source Yeast
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Code CSB-EP339711SXV
MSDS
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Source E.coli
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Code CSB-EP339711SXV-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP339711SXV
MSDS
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Source Baculovirus
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Code CSB-MP339711SXV
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
cig2
Uniprot No.
Alternative Names
cig2; cyc17; SPAPB2B4.03; G2/mitotic-specific cyclin cig2
Species
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Expression Region
1-411
Target Protein Sequence
MALYSISKPV GSKINKHSYQ DENTLVGKQA LSKGTEKTKL STNFEINLPR RTVLSDVSNV GKNNADEKDT KKAKRSFDES NLSTNEEADK PVESKFVKKL KVYSKNADPS VETLQKDRVS NVDDHLSSNP LMAEEYAPEI FEYIRKLDLK CLPNPKYMDQ QKELTWKMRE ILNEWLVEIH SNFCLMPETL YLAVNIIDRF LSRRSCSLSK FQLTGITALL IASKYEEVMC PSIQNFVYMT DGAFTVEDVC VAERYMLNVL NFDLSYPSPL NFLRKISQAE GYDAQTRTLG KYLTEIYLFD HDLLRYPMSK IAAAAMYLSR RLLRRGPWTP KLVESSGGYE EHELKEIAYI MLHYHNKPLE HKAFFQKYSS KRFLKASIFV HQLVRQRYSV NRTDDDDLQS EPSSSLTNDG H
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Essential for the control of the cell cycle at the G2/M and G1/S (mitosis) transition. Interacts with the cdc2 protein kinase to form MPF. Interaction with res2 promotes the phosphorylation of res1 and inhibits MBF-dependent gene transcription. Forms an autoregulating feedback-inhibition loop with MBF which is important for normal regulation of the cell cycle. G2/M cyclins accumulate steadily during G2 and are abruptly destroyed at mitosis. Negatively regulates conjugation via interacting with cell cycle 'start' genes. Degraded by skp1, pop1 and pop2 in the G2 and M phases of the cell cycle.
Subcellular Location
Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body.
Protein Families
Cyclin family, Cyclin AB subfamily
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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