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Recombinant Schizosaccharomyces pombe Mitosis inhibitor protein kinase wee1 (wee1), partial

Recombinant Schizosaccharomyces pombe Mitosis inhibitor protein kinase wee1 (wee1), partial

Code	CSB-YP357135SXV
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP357135SXV
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP357135SXV-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP357135SXV
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP357135SXV
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

wee1

Uniprot No.

P07527

Alternative Names

wee1; SPCC18B5.03Mitosis inhibitor protein kinase wee1; EC 2.7.10.2; P107 protein kinase homolog

Species

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Protein kinase that acts both on serines and on tyrosines. It acts as a dosage-dependent negative regulator of entry into mitosis (G2 to M transition). Phosphorylates and inhibits cdc2.

Gene References into Functions

Regulation of Wee1 localization around the spindle pole body during the G2/M transition is important for proper mitotic entry and progression. PMID: 21233285
in fission yeast Wee1 is the major inhibitory kinase regulating CDK activity in S phase. PMID: 26918805
This cell cycle arrest is dependent on the Wee1 tyrosine kinase inhibiting the key cell cycle regulator, CDK1/Cdc2. Mutant cells lacking Wee1 are not arrested at G2 upon glucose limitation and lose viability faster than the wild-type cells under glucose-depleted quiescent conditions, suggesting that this cell cycle arrest is required for extension of chronological lifespan PMID: 26804466
Wee1 phosphorylates Cdc2 Tyr15 which leads to replication slowdown in the fission yeast under nitrosative stress. PMID: 26006103
Dnt1 regulates G2/M transition and cytokinesis by downregulating Wee1 kinase. PMID: 24006256
Substitution of a conserved methionine residue to phenylalanine in Plo1, Orb5 and Wee1 kinases increased the sensitivities to ATP analogues. PMID: 23986474
Data show that the protein Skb1 interacted with both Cdr1 and the Cdr1 inhibitory target Wee1. PMID: 23615447
Sid2 activation midway through G2 phase promotes Fin1 phosphorylation and mitotic commitment throught pom1/Wee1 cell geometry network. PMID: 22684255
in fission yeast Cpc2/RACK1 positively regulates from the ribosome the mitotic onset by modulating both the protein levels and the activity of Wee1 PMID: 20974849
The changed division response (Cdr) kinases Cdr1 and Cdr2 are negative regulators of Wee1 and they are required for the Tra1-dependent alterations to Wee1 function. PMID: 20194963
findings demonstrate that G2 arrest induced by HIV-1 Vpr is also associated with increase in phosphorylation & amount of Wee1 in a Chk1/Cds1-independent manner; Rad24 appears to contribute to Vpr-induced G2 arrest by elevating Wee1 expression PMID: 16968670