Purity
Greater than 90% as determined by SDS-PAGE.
Alternative Names
clfB; SAR2709Clumping factor B; Fibrinogen receptor B; Fibrinogen-binding protein B
Species
Staphylococcus aureus (strain MRSA252)
Expression Region
45-542aa
Target Protein Sequence
SEQSNDTTQSSKNNASADSEKNNTIETPQLNTTANDTSDISANTNSANVDSTAKTMSTQTSNTTTTEPASTNETPQPTAIKDQATAAKMQDQTVPQEANSQVDNKTTNDANNIATNSELKNPQTLDLPQSSPQTISNAQGTSKPSVRTRAVRSLAVAEPVVNAADAKGTNVNDKVTASDFKLEKTAFDPNQSGNTFMAANFKVTGQVKSGDYFTAKLPDSVTGNGDVDYSNSNNTMPIADIKSTNGDVVAKATYDILTKTYTFVFTDYVNDKENINGQFSLPLFTDRAKAPKSGTYDANINIADEMFDNKITYNYSSPIAGIDKPNGANISSQIIGVDTASGQNTYKQTVFVNPKQRVLGNTWVYIKGYQDKIEESSGKVSATDTKLRIFEVNDTSKLSDSYYADPNDSNLKEVTGEFKDKISYKYDNVASINFGDINKTYVVLVEGHYDNTGKNLKTQVIQENIDPATGKDYSIFGWNNENVVRYGGGSADGDSAVN
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Tag Info
N-terminal 6xHis-SUMO-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The region for expressing recombinant Staphylococcus aureus (strain MRSA252) clfB contains amino acids 45-542. The expected molecular weight for the clfB protein is calculated to be 69.8 kDa. The clfB protein was expressed in e.coli. Fusion of the N-terminal 6xHis-SUMO tag into the clfB encoding gene fragment was conducted, allowing for easier detection and purification of the clfB protein in subsequent stages.
Staphylococcus aureus clumping factor B (ClfB) is a microbial surface component recognizing adhesive matrix molecules (MSCRAMM) crucial for bacterial adhesion and biofilm formation. The main function of ClfB involves binding to fibrinogen, facilitating S. aureus colonization on host tissues and medical implants. In infectious disease research, ClfB is a virulence factor and a potential target for therapeutic interventions against S. aureus infections. Investigating ClfB provides insights into bacterial pathogenesis, biofilm formation, and host-pathogen interactions. Additionally, understanding ClfB's role in biofilm-related infections contributes to antimicrobial strategies and the development of novel therapeutics to combat S. aureus-associated diseases, impacting medical and microbiological research.
