Purity
Greater than 90% as determined by SDS-PAGE.
Alternative Names
fabI; SAR0978Enoyl-[acyl-carrier-protein] reductase [NADPH] FabI; ENR; EC 1.3.1.39; NADPH-dependent enoyl-ACP reductase
Species
Staphylococcus aureus (strain MRSA252)
Expression Region
1-256aa
Target Protein Sequence
MLNLENKTYVIMGIANKRSIAFGVAKVLDQLGAKLVFTYRKERSRKELEKLLEQLNQPEAHLYQIDVQSDEEVINGFEQIGKDVGNIDGVYHSIAFANMEDLRGRFSETSREGFLLAQDISSYSLTIVAHEAKKLMPEGGSIVATTYLGGEFAVQNYNVMGVAKASLEANVKYLALDLGPDNIRVNAISAGPIRTLSAKGVGGFNTILKEIEERAPLKRNVDQVEVGKTAAYLLSDLSSGVTGENIHVDSGFHAIK
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length
Tag Info
N-terminal 6xHis-SUMO-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The region for expressing recombinant Staphylococcus aureus (strain MRSA252) fabI contains amino acids 1-256. The theoretical molecular weight of the fabI protein is 44 kDa. This protein is generated in a e.coli-based system. Fusion of the N-terminal 6xHis-SUMO tag into the fabI encoding gene fragment was conducted, allowing for easier detection and purification of the fabI protein in subsequent stages.
Staphylococcus aureus Enoyl-[acyl-carrier-protein] Reductase [NADPH] FabI is a key enzyme in bacterial fatty acid biosynthesis. Its main function involves catalyzing the reduction of enoyl-ACP, a critical step in fatty acid elongation. FabI is essential for bacterial membrane biogenesis, making it a target for antibiotic research. Investigating FabI provides insights into bacterial physiology, antimicrobial resistance, and antibiotic development. Understanding its structure and mechanism aids in designing novel antibacterial agents. FabI research extends to the broader field of bacterial metabolism, offering potential applications in developing strategies to combat Staphylococcus aureus infections and addressing challenges related to antibiotic resistance in clinical settings.
