Recombinant Streptococcus pneumoniae tRNA(Ile)-lysidine synthase

Product Type Recombinant Protein
Uniprot No. B1I6Y3
Species Streptococcus pneumoniae (strain Hungary19A-6)
Gene Names tilS
Protein Names Recommended name: tRNA(Ile)-lysidine synthase EC= 6.3.4.19 Alternative name(s): tRNA(Ile)-2-lysyl-cytidine synthase tRNA(Ile)-lysidine synthetase
Storage The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Function Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine.
Subcellular Location Cytoplasm
Protein Families TRNA(Ile)-lysidine synthase family
Database Links

KEGG: spv:SPH_0011

Product Details for Different Expression Systems

Product Name Recombinant Streptococcus pneumoniae tRNA(Ile)-lysidine synthase,Yeast
Code CSB-YP535423FNA
Size Pls inquiry other sizes
Source Yeast
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MREPDFLNHF LKKGYFKKHA KAVLALSGGL DSMFLFKVLS TYQKELEIEL ILAHVNHKQR IESDWEEKEL RKLAAEAELP IYISNFSGEF SEARARNFRY DFFQEVMKKT GATALVTAHH ADDQVETILM RLIRGTRLRY LSGIKEKQVV GEIEIIRPFL HFQKKDFPSI FHFEDTSNQE NHYFRNRIRN SYLPELEKEN PRFRDAILGI GNEILDYDLA IAELSNNINV EDLQQLFSYS ESTQRVLLQT YLNRFPDLNL TKAQFAEVQQ ILKSKSQYRH PIKNGYELIK EYQQFQICKI SPQADEEEDE FVLHYQNQVA YQGYLFSFGL PLEGESIQQI PVSRETSIHI RHRKTGDVLI QNGHRKKLRR LFIDLKIPME KRNSALIIEQ FGEIVSILGI ATNNLSKKTK NDIMNTVLYI EKIDR
Expression Region 1-425
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
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Product Name Recombinant Streptococcus pneumoniae tRNA(Ile)-lysidine synthase,E.coli
Code CSB-EP535423FNA
Size Pls inquiry other sizes
Source E.coli
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-SUMO-tagged and C-terminal Myc-tagged
N-terminal His-B2M-tagged and C-terminal Myc-tagged
N-terminal His-GST-tagged and C-terminal Myc-tagged
N-terminal His-tagged
N-terminal His-SUMO-tagged
N-terminal His-B2M-tagged
N-terminal His-GST-tagged
N-terminal GST-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MREPDFLNHF LKKGYFKKHA KAVLALSGGL DSMFLFKVLS TYQKELEIEL ILAHVNHKQR IESDWEEKEL RKLAAEAELP IYISNFSGEF SEARARNFRY DFFQEVMKKT GATALVTAHH ADDQVETILM RLIRGTRLRY LSGIKEKQVV GEIEIIRPFL HFQKKDFPSI FHFEDTSNQE NHYFRNRIRN SYLPELEKEN PRFRDAILGI GNEILDYDLA IAELSNNINV EDLQQLFSYS ESTQRVLLQT YLNRFPDLNL TKAQFAEVQQ ILKSKSQYRH PIKNGYELIK EYQQFQICKI SPQADEEEDE FVLHYQNQVA YQGYLFSFGL PLEGESIQQI PVSRETSIHI RHRKTGDVLI QNGHRKKLRR LFIDLKIPME KRNSALIIEQ FGEIVSILGI ATNNLSKKTK NDIMNTVLYI EKIDR
Expression Region 1-425
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat
Product Name Recombinant Streptococcus pneumoniae tRNA(Ile)-lysidine synthase,Baculovirus
Code CSB-BP535423FNA
Size Pls inquiry other sizes
Source Baculovirus
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MREPDFLNHF LKKGYFKKHA KAVLALSGGL DSMFLFKVLS TYQKELEIEL ILAHVNHKQR IESDWEEKEL RKLAAEAELP IYISNFSGEF SEARARNFRY DFFQEVMKKT GATALVTAHH ADDQVETILM RLIRGTRLRY LSGIKEKQVV GEIEIIRPFL HFQKKDFPSI FHFEDTSNQE NHYFRNRIRN SYLPELEKEN PRFRDAILGI GNEILDYDLA IAELSNNINV EDLQQLFSYS ESTQRVLLQT YLNRFPDLNL TKAQFAEVQQ ILKSKSQYRH PIKNGYELIK EYQQFQICKI SPQADEEEDE FVLHYQNQVA YQGYLFSFGL PLEGESIQQI PVSRETSIHI RHRKTGDVLI QNGHRKKLRR LFIDLKIPME KRNSALIIEQ FGEIVSILGI ATNNLSKKTK NDIMNTVLYI EKIDR
Expression Region 1-425
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat
Product Name Recombinant Streptococcus pneumoniae tRNA(Ile)-lysidine synthase,Mammalian cell
Code CSB-MP535423FNA
Size Pls inquiry other sizes
Source Mammalian cell
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MREPDFLNHF LKKGYFKKHA KAVLALSGGL DSMFLFKVLS TYQKELEIEL ILAHVNHKQR IESDWEEKEL RKLAAEAELP IYISNFSGEF SEARARNFRY DFFQEVMKKT GATALVTAHH ADDQVETILM RLIRGTRLRY LSGIKEKQVV GEIEIIRPFL HFQKKDFPSI FHFEDTSNQE NHYFRNRIRN SYLPELEKEN PRFRDAILGI GNEILDYDLA IAELSNNINV EDLQQLFSYS ESTQRVLLQT YLNRFPDLNL TKAQFAEVQQ ILKSKSQYRH PIKNGYELIK EYQQFQICKI SPQADEEEDE FVLHYQNQVA YQGYLFSFGL PLEGESIQQI PVSRETSIHI RHRKTGDVLI QNGHRKKLRR LFIDLKIPME KRNSALIIEQ FGEIVSILGI ATNNLSKKTK NDIMNTVLYI EKIDR
Expression Region 1-425
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat

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