Recombinant Streptococcus pyogenes serotype M12 50S ribosomal protein L2(rplB)

Code CSB-YP629474SAAV
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Source Yeast
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Code CSB-EP629474SAAV
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Source E.coli
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Code CSB-EP629474SAAV-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP629474SAAV
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Source Baculovirus
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Code CSB-MP629474SAAV
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names rplB
Uniprot No. Q1JP14
Alternative Names rplB; MGAS9429_Spy0047; 50S ribosomal protein L2
Species Streptococcus pyogenes serotype M12 (strain MGAS9429)
Expression Region 1-277
Target Protein Sequence MGIKVYKPTT NGRRNMTSLD FAEITTSTPE KSLLVSLKSK AGRNNNGRIT VRHQGGGHKR HYRLIDFKRN KDGVEAVVKT IEYDPNRTAN IALVHYTDGV KAYIIAPKGL EVGQRIVSGP DADIKVGNAL PLANIPVGTV VHNIELKPGK GGELVRAAGA SAQVLGQEGK YVLVRLQSGE VRMILGTCRA TIGTVGNEQQ SLVNIGKAGR SRWKGIRPTV RGSVMNPNDH PHGGGEGKAP VGRKAPSTPW GKPALGLKTR NKKAKSDKLI VRRRNEK
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

Target Data

Function One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity; this is somewhat controversial. Makes several contacts with the 16S rRNA in the 70S ribosome.
Protein Families Universal ribosomal protein uL2 family
Database Links

KEGG: spk:MGAS9429_Spy0047

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