Recombinant Synechococcus elongatus Uncharacterized protein CcmO (ccmO)

Code CSB-YP341598FPY
MSDS
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Source Yeast
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Code CSB-EP341598FPY
MSDS
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Source E.coli
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Code CSB-EP341598FPY-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP341598FPY
MSDS
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Source Baculovirus
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Code CSB-MP341598FPY
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ccmO
Uniprot No.
Alternative Names
ccmO; Synpcc7942_1425Uncharacterized protein CcmO
Species
Synechococcus elongatus (strain PCC 7942) (Anacystis nidulans R2)
Expression Region
30-276
Target Protein Sequence
G TADMMLKSAD VTLIGYEKTG SGFCTAIIRG GYADIKLALE AGVATARQFE QYVSSTILPR PQGNLEAVLP ISRRLSQEAM ATRSHQNVGA IGLIETNGFP ALVGAADAML KSANVKLICY EKTGSGLCTA IVQGTVSNVT VAVEAGMYAA ERIGQLNAIM VIPRPLDDLM DSLPEPQSDS EAAQPLQLPL RVREKQPLLE LPELERQPIA IEAPRLLAEE RQSALELAQE TPLAEPLELP NPRDDQ
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Required for formation of the carboxysome, a polyhedral inclusion where RuBisCO (ribulose bisphosphate carboxylase, rbcL-rbcS) is sequestered. Required for recruitment of major shell protein CcmK2 to the pre-carboxysome. Suggested to be a carboxysome shell protein, but it is not detected in gels, mass spectrometry or by protein sequencing (Probable).; Beta-carboxysome assembly initiates when soluble RuBisCO is condensed into a liquid matrix in a pre-carboxysome by the RbcS-like domains of probably both CcmM58 and CcmM35. CcmN interacts with the N-terminus of CcmM58, and then recruits the CcmK2 major shell protein via CcmN's encapsulation peptide. Shell formation requires CcmK proteins and CcmO. CcmL caps the otherwise elongated carboxysome. Once fully encapsulated carboxysomes are formed, they migrate within the cell probably via interactions with the cytoskeleton.
Subcellular Location
Carboxysome.
Protein Families
Bacterial microcompartments protein family
Database Links
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