Recombinant Type-1 restriction enzyme EcoEI specificity protein(hsdS)

Product Type Recombinant Protein
Uniprot No. P19705
Species Escherichia coli
Gene Names hsdS
Protein Names Recommended name: Type-1 restriction enzyme EcoEI specificity protein Short name= S.EcoEI Alternative name(s): Type I restriction enzyme EcoEI specificity protein Short name= S protein
Storage The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Function The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to the same target sequence but cutting the DNA some distance from this site. Whether the DNA is cut or modified depends on the methylation state of the target sequence. When the target site is unmodified, the DNA is cut. When the target site is hemimethylated, the complex acts as a maintenance MTase modifying the DNA so that both strands become methylated. Subunit S dictates DNA sequences specificity. The EcoEI enzyme recognizes 5'-GAGN(7)ATGC-3'.
Protein Families Type-I restriction system S methylase family

Product Details for Different Expression Systems

Product Name Recombinant Type-1 restriction enzyme EcoEI specificity protein(hsdS),Yeast
Code CSB-YP322554ENL
Size Pls inquiry other sizes
Source Yeast
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MAVEKLITDH IDIWSSALQT RSMAGRGSNG KIDLYGIKKL RELILELAVR GKLVPQDPND EPASELLKRI AAEKTELVKQ GKIKKQKPLL RISEDEKPFE LPEGWEWITL SEIATINPKI EVTDDEQEIS FVPMPCISTR FDGAHDQEIK KWGEVKKGYT HFADGDIALA KITPCFENSK AVIFKGLKGG VGVGTTELHV ARPISSELNL QYILLNIKSP HYLSMGESMM TGSAGQKRVP RSFFENYPIP FPPNTEQARI VGTFSKLMFL CDQLEQQSLT SLDAHQQLVE TLLATLTDSQ NAEELAENWA RISQYFDTLF TTEASIDALK QTILQLAVMG KLVSQDPNDE PASELLKRVE QEKVQLVKEG KIKKQKPLPP VSDDEKPFEL PIGWEWCRIG EIIANMDAGW SPACSPEPSP NEDIWGVLKT TAVQSLEYRE QENKTLPNSK LPRPQYEVHD GDILVTRAGP KNRVGVSCLV EKTRSKLMIS DKIIRFHLIS DDISAKYISL CLNRGVTADY LEASKSGMAE SQMNISQENL RSAPIALPPT AIQLKVISTI EDFFKVCDQL KSRLQSAQQT QLHLADALTD AALN
Expression Region 1-594
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
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Product Name Recombinant Type-1 restriction enzyme EcoEI specificity protein(hsdS),E.coli
Code CSB-EP322554ENL
Size Pls inquiry other sizes
Source E.coli
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-SUMO-tagged and C-terminal Myc-tagged
N-terminal His-B2M-tagged and C-terminal Myc-tagged
N-terminal His-GST-tagged and C-terminal Myc-tagged
N-terminal His-tagged
N-terminal His-SUMO-tagged
N-terminal His-B2M-tagged
N-terminal His-GST-tagged
N-terminal GST-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MAVEKLITDH IDIWSSALQT RSMAGRGSNG KIDLYGIKKL RELILELAVR GKLVPQDPND EPASELLKRI AAEKTELVKQ GKIKKQKPLL RISEDEKPFE LPEGWEWITL SEIATINPKI EVTDDEQEIS FVPMPCISTR FDGAHDQEIK KWGEVKKGYT HFADGDIALA KITPCFENSK AVIFKGLKGG VGVGTTELHV ARPISSELNL QYILLNIKSP HYLSMGESMM TGSAGQKRVP RSFFENYPIP FPPNTEQARI VGTFSKLMFL CDQLEQQSLT SLDAHQQLVE TLLATLTDSQ NAEELAENWA RISQYFDTLF TTEASIDALK QTILQLAVMG KLVSQDPNDE PASELLKRVE QEKVQLVKEG KIKKQKPLPP VSDDEKPFEL PIGWEWCRIG EIIANMDAGW SPACSPEPSP NEDIWGVLKT TAVQSLEYRE QENKTLPNSK LPRPQYEVHD GDILVTRAGP KNRVGVSCLV EKTRSKLMIS DKIIRFHLIS DDISAKYISL CLNRGVTADY LEASKSGMAE SQMNISQENL RSAPIALPPT AIQLKVISTI EDFFKVCDQL KSRLQSAQQT QLHLADALTD AALN
Expression Region 1-594
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
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Product Name Recombinant Type-1 restriction enzyme EcoEI specificity protein(hsdS),Baculovirus
Code CSB-BP322554ENL
Size Pls inquiry other sizes
Source Baculovirus
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MAVEKLITDH IDIWSSALQT RSMAGRGSNG KIDLYGIKKL RELILELAVR GKLVPQDPND EPASELLKRI AAEKTELVKQ GKIKKQKPLL RISEDEKPFE LPEGWEWITL SEIATINPKI EVTDDEQEIS FVPMPCISTR FDGAHDQEIK KWGEVKKGYT HFADGDIALA KITPCFENSK AVIFKGLKGG VGVGTTELHV ARPISSELNL QYILLNIKSP HYLSMGESMM TGSAGQKRVP RSFFENYPIP FPPNTEQARI VGTFSKLMFL CDQLEQQSLT SLDAHQQLVE TLLATLTDSQ NAEELAENWA RISQYFDTLF TTEASIDALK QTILQLAVMG KLVSQDPNDE PASELLKRVE QEKVQLVKEG KIKKQKPLPP VSDDEKPFEL PIGWEWCRIG EIIANMDAGW SPACSPEPSP NEDIWGVLKT TAVQSLEYRE QENKTLPNSK LPRPQYEVHD GDILVTRAGP KNRVGVSCLV EKTRSKLMIS DKIIRFHLIS DDISAKYISL CLNRGVTADY LEASKSGMAE SQMNISQENL RSAPIALPPT AIQLKVISTI EDFFKVCDQL KSRLQSAQQT QLHLADALTD AALN
Expression Region 1-594
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat
Product Name Recombinant Type-1 restriction enzyme EcoEI specificity protein(hsdS),Mammalian cell
Code CSB-MP322554ENL
Size Pls inquiry other sizes
Source Mammalian cell
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MAVEKLITDH IDIWSSALQT RSMAGRGSNG KIDLYGIKKL RELILELAVR GKLVPQDPND EPASELLKRI AAEKTELVKQ GKIKKQKPLL RISEDEKPFE LPEGWEWITL SEIATINPKI EVTDDEQEIS FVPMPCISTR FDGAHDQEIK KWGEVKKGYT HFADGDIALA KITPCFENSK AVIFKGLKGG VGVGTTELHV ARPISSELNL QYILLNIKSP HYLSMGESMM TGSAGQKRVP RSFFENYPIP FPPNTEQARI VGTFSKLMFL CDQLEQQSLT SLDAHQQLVE TLLATLTDSQ NAEELAENWA RISQYFDTLF TTEASIDALK QTILQLAVMG KLVSQDPNDE PASELLKRVE QEKVQLVKEG KIKKQKPLPP VSDDEKPFEL PIGWEWCRIG EIIANMDAGW SPACSPEPSP NEDIWGVLKT TAVQSLEYRE QENKTLPNSK LPRPQYEVHD GDILVTRAGP KNRVGVSCLV EKTRSKLMIS DKIIRFHLIS DDISAKYISL CLNRGVTADY LEASKSGMAE SQMNISQENL RSAPIALPPT AIQLKVISTI EDFFKVCDQL KSRLQSAQQT QLHLADALTD AALN
Expression Region 1-594
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat

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