Recombinant Type-1 restriction enzyme EcoprrI specificity protein(prrB)

Product Type Recombinant Protein
Uniprot No. P17222
Species Escherichia coli
Gene Names prrB
Protein Names Recommended name: Type-1 restriction enzyme EcoprrI specificity protein Short name= S.EcoprrI Alternative name(s): Type I restriction enzyme EcoprrI specificity protein Short name= S protein
Storage The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Function The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to the same target sequence but cutting the DNA some distance from this site. Whether the DNA is cut or modified depends on the methylation state of the target sequence. When the target site is unmodified, the DNA is cut. When the target site is hemimethylated, the complex acts as a maintenance MTase modifying the DNA so that both strands become methylated. Subunit S dictates DNA sequences specificity. The EcoprrI enzyme recognizes 5'-CCAN(7)ATGC-3'.
Protein Families Type-I restriction system S methylase family

Product Details for Different Expression Systems

Product Name Recombinant Type-1 restriction enzyme EcoprrI specificity protein(prrB),Yeast
Code CSB-YP322344ENL
Size Pls inquiry other sizes
Source Yeast
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MSELSYLEKL MDGVEVEWLP LSKVFNLRNG YTPSKTKKEF WANGDIPWFR MDDIRENGRI LGSSLQKISS CAVKGGKLFP ENSILISTSA TIGEHALITV PHLANQRFTC LALKESYADC FDIKFLFYYC FSLAEWCRKN TTMSSFASVD MDGFKKFLIP RPCPDNPEKS LAIQSEIVRI LDKFSALTAE LTAELTAELS MRKKQYNYYR DQLLSFKEDE VEGKRKTLGE IMKMRAGQHI SAHNIIERKE ESYIYPCFGG NGIRGYVKEK SHDGEHLLIG RQGALCGNVQ RMKGQFYATE HAVVVSVMPG INIDWAFHML TAMNLNQYAS KSAQPGLAVG KLQELKLFVP SIERQIYIAA ILDKFDTLTN SITEVSRVKS SCARNSTNII EICYLVSRSR K
Expression Region 1-401
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
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Product Name Recombinant Type-1 restriction enzyme EcoprrI specificity protein(prrB),E.coli
Code CSB-EP322344ENL
Size Pls inquiry other sizes
Source E.coli
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-SUMO-tagged and C-terminal Myc-tagged
N-terminal His-B2M-tagged and C-terminal Myc-tagged
N-terminal His-GST-tagged and C-terminal Myc-tagged
N-terminal His-tagged
N-terminal His-SUMO-tagged
N-terminal His-B2M-tagged
N-terminal His-GST-tagged
N-terminal GST-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MSELSYLEKL MDGVEVEWLP LSKVFNLRNG YTPSKTKKEF WANGDIPWFR MDDIRENGRI LGSSLQKISS CAVKGGKLFP ENSILISTSA TIGEHALITV PHLANQRFTC LALKESYADC FDIKFLFYYC FSLAEWCRKN TTMSSFASVD MDGFKKFLIP RPCPDNPEKS LAIQSEIVRI LDKFSALTAE LTAELTAELS MRKKQYNYYR DQLLSFKEDE VEGKRKTLGE IMKMRAGQHI SAHNIIERKE ESYIYPCFGG NGIRGYVKEK SHDGEHLLIG RQGALCGNVQ RMKGQFYATE HAVVVSVMPG INIDWAFHML TAMNLNQYAS KSAQPGLAVG KLQELKLFVP SIERQIYIAA ILDKFDTLTN SITEVSRVKS SCARNSTNII EICYLVSRSR K
Expression Region 1-401
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat
Product Name Recombinant Type-1 restriction enzyme EcoprrI specificity protein(prrB),Baculovirus
Code CSB-BP322344ENL
Size Pls inquiry other sizes
Source Baculovirus
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MSELSYLEKL MDGVEVEWLP LSKVFNLRNG YTPSKTKKEF WANGDIPWFR MDDIRENGRI LGSSLQKISS CAVKGGKLFP ENSILISTSA TIGEHALITV PHLANQRFTC LALKESYADC FDIKFLFYYC FSLAEWCRKN TTMSSFASVD MDGFKKFLIP RPCPDNPEKS LAIQSEIVRI LDKFSALTAE LTAELTAELS MRKKQYNYYR DQLLSFKEDE VEGKRKTLGE IMKMRAGQHI SAHNIIERKE ESYIYPCFGG NGIRGYVKEK SHDGEHLLIG RQGALCGNVQ RMKGQFYATE HAVVVSVMPG INIDWAFHML TAMNLNQYAS KSAQPGLAVG KLQELKLFVP SIERQIYIAA ILDKFDTLTN SITEVSRVKS SCARNSTNII EICYLVSRSR K
Expression Region 1-401
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat
Product Name Recombinant Type-1 restriction enzyme EcoprrI specificity protein(prrB),Mammalian cell
Code CSB-MP322344ENL
Size Pls inquiry other sizes
Source Mammalian cell
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MSELSYLEKL MDGVEVEWLP LSKVFNLRNG YTPSKTKKEF WANGDIPWFR MDDIRENGRI LGSSLQKISS CAVKGGKLFP ENSILISTSA TIGEHALITV PHLANQRFTC LALKESYADC FDIKFLFYYC FSLAEWCRKN TTMSSFASVD MDGFKKFLIP RPCPDNPEKS LAIQSEIVRI LDKFSALTAE LTAELTAELS MRKKQYNYYR DQLLSFKEDE VEGKRKTLGE IMKMRAGQHI SAHNIIERKE ESYIYPCFGG NGIRGYVKEK SHDGEHLLIG RQGALCGNVQ RMKGQFYATE HAVVVSVMPG INIDWAFHML TAMNLNQYAS KSAQPGLAVG KLQELKLFVP SIERQIYIAA ILDKFDTLTN SITEVSRVKS SCARNSTNII EICYLVSRSR K
Expression Region 1-401
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat

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