Recombinant Type-1 restriction enzyme EcoprrI specificity protein (prrB)

Code CSB-YP322344ENL
MSDS
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Source Yeast
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Code CSB-EP322344ENL
MSDS
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Source E.coli
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Code CSB-EP322344ENL-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP322344ENL
MSDS
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Source Baculovirus
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Code CSB-MP322344ENL
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
prrB
Uniprot No.
Alternative Names
prrB; Type-1 restriction enzyme EcoprrI specificity protein; S.EcoprrI; Type I restriction enzyme EcoprrI specificity protein; S protein
Species
Escherichia coli
Expression Region
1-401
Target Protein Sequence
MSELSYLEKL MDGVEVEWLP LSKVFNLRNG YTPSKTKKEF WANGDIPWFR MDDIRENGRI LGSSLQKISS CAVKGGKLFP ENSILISTSA TIGEHALITV PHLANQRFTC LALKESYADC FDIKFLFYYC FSLAEWCRKN TTMSSFASVD MDGFKKFLIP RPCPDNPEKS LAIQSEIVRI LDKFSALTAE LTAELTAELS MRKKQYNYYR DQLLSFKEDE VEGKRKTLGE IMKMRAGQHI SAHNIIERKE ESYIYPCFGG NGIRGYVKEK SHDGEHLLIG RQGALCGNVQ RMKGQFYATE HAVVVSVMPG INIDWAFHML TAMNLNQYAS KSAQPGLAVG KLQELKLFVP SIERQIYIAA ILDKFDTLTN SITEVSRVKS SCARNSTNII EICYLVSRSR K
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to the same target sequence but cutting the DNA some distance from this site. Whether the DNA is cut or modified depends on the methylation state of the target sequence. When the target site is unmodified, the DNA is cut. When the target site is hemimethylated, the complex acts as a maintenance MTase modifying the DNA so that both strands become methylated. Subunit S dictates DNA sequences specificity. The EcoprrI enzyme recognizes 5'-CCAN(7)ATGC-3'.
Protein Families
Type-I restriction system S methylase family
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