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Recombinant Uncharacterized protein F54F2.2, isoform a (F54F2.2/F54F2.3/F54F2.4), partial

Recombinant Uncharacterized protein F54F2.2, isoform a (F54F2.2/F54F2.3/F54F2.4), partial

Code	CSB-YP335940CXY
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP335940CXY
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP335940CXY-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP335940CXY
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP335940CXY
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

F54F2.2/F54F2.3/F54F2.4

Uniprot No.

P34447

Alternative Names

zfp-1; F54F2.2Zinc finger protein zfp-1; Protein AF-10 homolog

Species

Caenorhabditis elegans

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Recruits the histone methyltransferase dot-1.1 to chromatin to methylate 'Lys-79' of histone H3 and activate transcription. Recognizes and binds histone H3 methylated at 'Lys-4' (H3K4me) at the promoters of target genes. During stress, the zfp-1-dot-1.1 complex also plays a role in the deubiquitination of histone H2B sites, which negatively modulates the RNA polymerase II-induced transcription of highly expressed genes. In response to stress, binds to the pdk-1 promoter to negatively regulate pdk-1 expression, which negatively modulates daf-16/FOXO-mediated gene expression. Thus, most likely via this mechanism, in response to stress, it confers a protective role against neuronal necrosis. Plays a role in Insulin/IGF-1-like signaling (IIS)- and diet restriction-mediated lifespan extension by controlling daf-16/FOXO and pha-4/FOXA recruitment to target promoters. May negatively regulate the expression of genes required for vulval development. May play a role in axon guidance in D-type motor neurons. May suppress sensitivity to RNAi.; Required for migration of HSN motor neurons during embryogenesis.

Gene References into Functions

the coordinated activities of DAF-16, ZFP-1, and endogenous RNAi contribute to gene regulation during development to ensure proper neuronal positioning PMID: 24558261
ZFP-1 localization peaks overlap with H3K4 methylation-enriched promoters of actively expressed genes genomewide and that H3K4 methylation is important for ZFP-1 localization to promoters in the embryo. PMID: 23263989
We found that the function of ZFP-1 in modulating pdk-1 transcription was important for the extended lifespan of the age-1(hx546) reduction-of-function PI3 kinase mutant. PMID: 21980302

Subcellular Location

Nucleus. Chromosome.

Tissue Specificity

Isoform a: Expressed at high levels in maturing oocytes, but at low levels in the rest of the germ line (at protein level). Isoform a: Not expressed in the pharynx, germ line and tail. Isoform c: Not expressed in the germ line (at protein level). Isoform

Database Links

STRING: 6239.F54F2.2a.3

UniGene: Cel.22669

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