Recombinant Vaccinia virus Protein E3 (E3L)

Code CSB-YP321992VAI
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Source Yeast
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Code CSB-EP321992VAI
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Source E.coli
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Code CSB-EP321992VAI-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP321992VAI
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Source Baculovirus
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Code CSB-MP321992VAI
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
VACWR059
Uniprot No.
Alternative Names
VACWR059; E3LProtein E3; p25
Species
Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))
Expression Region
1-190
Target Protein Sequence
MSKIYIDERS NAEIVCEAIK TIGIEGATAA QLTRQLNMEK REVNKALYDL QRSAMVYSSD DIPPRWFMTT EADKPDADAM ADVIIDDVSR EKSMREDHKS FDDVIPAKKI IDWKGANPVT VINEYCQITR RDWSFRIESV GPSNSPTFYA CVDIDGRVFD KADGKSKRDA KNNAAKLAVD KLLGYVIIRF
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Plays a role in the inhibition of multiple cellular antiviral responses activated by dsRNA, such as inhibition of PKR activation, apoptosis, and IFN-mediated antiviral activities. Blocks also the phosphorylation and subsequent activation of IRF3 and IRF7 kinases that are required for interferon-alpha gene expression. Inhibits NF-kappa-B activation and the ubiquitin-like protein ISG15, which is an early antiviral protein. The binding with host ISG15 subsequently blocks host ISGylation. Inhibits ZBP1-dependent necroptosis via interaction with host ZBP1.
Gene References into Functions
  1. Vaccinia virus E3L protein complements the human adenovirus type 5 virus-associated RNAi defect. PMID: 26196231
  2. Ectopic expression of vaccinia virus E3 and K3 cannot rescue ectromelia virus replication in rabbit RK13 cells. PMID: 25734776
  3. examination of a set of E3L mutants showed that residues essential for dsRNA binding are not essential for the biological functions of E3 protein, such as inhibition of protein kinase R activation, suppression of cytokine expression, and apoptosis PMID: 25740987
  4. The amino terminus of the vaccinia virus E3 protein is necessary to inhibit the interferon response. PMID: 22419806
  5. There are commonalities but also functional differences in the roles of NS1 and E3 as inhibitors of the innate antiviral response. PMID: 22174864
  6. The authors demonstrate that vaccinia virus E3 interacts with SUMO1 through a small ubiquitin-like modifier (SUMO)-interacting motif (SIM). PMID: 21957283
  7. investigated whether the E3 Z-DNA-binding domain functions by sequestering cytoplasmic dsDNA thereby preventing the induction of type I interferon PMID: 20519457
  8. the single-cycle yield of delta-E3L virus was increased by nearly 2 log10 in PKR-deficient cells over the impaired growth in PKR-sufficient cells. PMID: 17959656
  9. The Z alpha domain of vaccinia E3L acquires ability to convert B-DNA to Z-DNA by mutating amino acid residues in this region. PMID: 17984076
  10. VV E3L can mediate strong inhibition activity against all human type I and type II IFNs, mainly through modulation of the PKR pathway in Huh7 cells. PMID: 18502465
  11. The dsRNA-binding domain of E3L suffices to prevent activation of the innate immune response. PMID: 18715932
  12. Vaccinia virus E3 protein can antagonize induction of multiple cytokines through several distinct but overlapping pathways. PMID: 19369349

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Protein Families
Poxviridae E3 protein family
Database Links

KEGG: vg:3707592

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