Recombinant Vaccinia virus Serine proteinase inhibitor 3 (SPI-3)

Code CSB-YP322434VAI
MSDS
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Source Yeast
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Code CSB-EP322434VAI
MSDS
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Source E.coli
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Code CSB-EP322434VAI-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP322434VAI
MSDS
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Source Baculovirus
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Code CSB-MP322434VAI
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SPI-3
Uniprot No.
Alternative Names
SPI-3; VACWR033; K2L; Protein K2; Serine proteinase inhibitor 3
Species
Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))
Expression Region
16-369
Target Protein Sequence
YRLQG FTNAGIVAYK NIQDDNIVFS PFGYSFSMFM SLLPASGNTR IELLKTMDLR KRDLGPAFTE LISGLAKLKT SKYTYTDLTY QSFVDNTVCI KPSYYQQYHR FGLYRLNFRR DAVNKINSIV ERRSGMSNVV DSNMLDNNTL WAIINTIYFK GIWQYPFDIT KTRNASFTNK YGTKTVPMMN VVTKLQGNTI TIDDEEYDMV RLPYKDANIS MYLAIGDNMT HFTDSITAAK LDYWSFQLGN KVYNLKLPKF SIENKRDIKS IAEMMAPSMF NPDNASFKHM TRDPLYIYKM FQNAKIDVDE QGTVAEASTI MVATARSSPE KLEFNTPFVF IIRHDITGFI LFMGKVESP
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Prevents cell to cell fusion via its interaction with A56 protein. The A56-K2 complex associates with components of the entry fusion complex (EFC) presumably to avoid superinfection and syncytium formation.
Gene References into Functions
  1. The SPI-3/A56 complex on the infected cell surface therefore appears to reduce the entry of virions into infected cells. PMID: 18760436
  2. The authors have shown that A56/K2 is sufficient to prevent virus entry and fusion as well as formation of syncytia through interaction with the entry/fusion complex. PMID: 19036815
Subcellular Location
Virion membrane; Peripheral membrane protein. Host cell membrane; Peripheral membrane protein; Extracellular side. Note=Component of extracellular enveloped virus (EEV) but not intracellular mature virus (IMV). Anchored to the surface of the outermost membrane of EEV via its interaction with A56 protein.
Protein Families
Serpin family, Poxviruses subfamily
Database Links

KEGG: vg:3707648

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