Recombinant Vaccinia virus Serine/threonine-protein kinase 1 (VPK1)

Code CSB-YP322323VAI
MSDS
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Source Yeast
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Code CSB-EP322323VAI
MSDS
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Source E.coli
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Code CSB-EP322323VAI-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP322323VAI
MSDS
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Source Baculovirus
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Code CSB-MP322323VAI
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
VPK1
Uniprot No.
Alternative Names
VPK1; VACWR183; B1RB1 kinase; Serine/threonine-protein kinase 1; EC 2.7.11.1; Vaccinia protein kinase 1
Species
Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))
Expression Region
1-300
Target Protein Sequence
MNFQGLVLTD NCKNQWVVGP LIGKGGFGSI YTTNDNNYVV KIEPKANGSL FTEQAFYTRV LKPSVIEEWK KSHNIKHVGL ITCKAFGLYK SINVEYRFLV INRLGADLDA VIRANNNRLP KRSVMLIGIE ILNTIQFMHE QGYSHGDIKA SNIVLDQIDK NKLYLVDYGL VSKFMSNGEH VPFIRNPNKM DNGTLEFTPI DSHKGYVVSR RGDLETLGYC MIRWLGGILP WTKISETKNC ALVSATKQKY VNNTATLLMT SLQYAPRELL QYITMVNSLT YFEEPNYDEF RHILMQGVYY
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Essential serine/threonine-protein kinase that plays different role in the viral life cycle. Phosphorylates the host small ribosomal protein RACK1 thereby customizing the ribosomes to a state optimal for viral mRNAs (which contain poly-A leaders) but not for host mRNAs. Facilitates viral DNA replication by inhibiting host BANF1, a cellular host defense responsive to foreign DNA. Phosphorylates host BANF1 on serine and threonine residues; this leads to BANF1 relocalization to the cytoplasm, loss of dimerization and impaired DNA binding activity. Indeed, BANF1 activity depends on its DNA-binding property which is blocked by VPK1-mediated phosphorylation. Required for viral intermediate genes expression, probably by inhibiting host BANF1. Modulates cellular responses via host JUN by two different mechanisms, either by direct phosphorylation or by modulation of upstream JIP1-MAPK complexes. Seems to participate in the accumulation/processing of late proteins and thus in virion maturation.
Gene References into Functions
  1. trans-kingdom mimicry underlies ribosome customization by a poxvirus kinase, vaccinia virus B1 PMID: 28636603
  2. These data support a model in which vaccinia virus B1 and human VRK2 share additional substrates important for the replication of cytoplasmic poxviruses. PMID: 28515294
  3. Vaccinia virus B1 kinase is needed for multiple critical junctures in the poxviral life cycle in a manner that is both dependent on and independent of BAF. PMID: 26223647
  4. The decrease in vaccinia virus transcription caused by loss of B1 kinase can be rescued by depletion of BAF. PMID: 23891157
  5. We now show that BAF acts as a potent inhibitor of poxvirus replication unless its DNA-binding activity is blocked by B1-mediated phosphorylation. PMID: 18005698

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Subcellular Location
Virion. Host cytoplasm.
Protein Families
Protein kinase superfamily, Ser/Thr protein kinase family, Poxviruses subfamily
Database Links

KEGG: vg:3707654

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