Recombinant Vaccinia virus Serine/threonine-protein kinase 1(VPK1)

Code CSB-YP322323VAI
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Source Yeast
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Code CSB-EP322323VAI
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Source E.coli
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Code CSB-EP322323VAI-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP322323VAI
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Source Baculovirus
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Code CSB-MP322323VAI
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names VPK1
Uniprot No. P16913
Alternative Names VPK1; VACWR183; B1RB1 kinase; Serine/threonine-protein kinase 1; EC 2.7.11.1; Vaccinia protein kinase 1
Species Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))
Expression Region 1-300
Target Protein Sequence MNFQGLVLTD NCKNQWVVGP LIGKGGFGSI YTTNDNNYVV KIEPKANGSL FTEQAFYTRV LKPSVIEEWK KSHNIKHVGL ITCKAFGLYK SINVEYRFLV INRLGADLDA VIRANNNRLP KRSVMLIGIE ILNTIQFMHE QGYSHGDIKA SNIVLDQIDK NKLYLVDYGL VSKFMSNGEH VPFIRNPNKM DNGTLEFTPI DSHKGYVVSR RGDLETLGYC MIRWLGGILP WTKISETKNC ALVSATKQKY VNNTATLLMT SLQYAPRELL QYITMVNSLT YFEEPNYDEF RHILMQGVYY
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

Target Data

Function Essential serine/threonine-protein kinase that plays different role in the viral life cycle
Gene References into Functions
  1. trans-kingdom mimicry underlies ribosome customization by a poxvirus kinase, vaccinia virus B1 PMID: 28636603
  2. These data support a model in which vaccinia virus B1 and human VRK2 share additional substrates important for the replication of cytoplasmic poxviruses. PMID: 28515294
  3. Vaccinia virus B1 kinase is needed for multiple critical junctures in the poxviral life cycle in a manner that is both dependent on and independent of BAF. PMID: 26223647
  4. The decrease in vaccinia virus transcription caused by loss of B1 kinase can be rescued by depletion of BAF. PMID: 23891157
  5. We now show that BAF acts as a potent inhibitor of poxvirus replication unless its DNA-binding activity is blocked by B1-mediated phosphorylation. PMID: 18005698

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Subcellular Location Virion, Host cytoplasm
Protein Families Protein kinase superfamily, Ser/Thr protein kinase family, Poxviruses subfamily
Database Links

KEGG: vg:3707654

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