Recombinant Vibrio anguillarum Flagellin B (flaB)

Code CSB-YP684930VEV
MSDS
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Source Yeast
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Code CSB-EP684930VEV
MSDS
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Source E.coli
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Code CSB-EP684930VEV-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP684930VEV
MSDS
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Source Baculovirus
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Code CSB-MP684930VEV
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
flaB
Uniprot No.
Alternative Names
flaBFlagellin B
Species
Vibrio anguillarum (Listonella anguillarum)
Expression Region
2-376
Target Protein Sequence
AINVSTNVS AMTAQRYLNN AADGTQKSME RLSSGYKINS ARDDAAGLQI SNRLTSQSRG LDMAVRNAND GISIAQTAEG AMNETTNILQ RMRDLSLQSA NGSNSSSERQ AIQEEVSALN DELNRIAETT SFGGNKLLNG SFGNKSFQIG ADSGEAVMLS MSDMRSDTKA MGGKSYVATN GKAPDWSVTN ATDLTLSYTD KQGEAREVTI NAKAGDDLEE VATYINGQNG DIKASVGDEG KLQLFAANQK VSSDVTIGGG LGTEIGFAAG KDVTVKDINV TTVGGSQEAV ALIDGALKAV DSQRASLGAF QNRFGHAISN LDNINENVNA SRSRIKDTDY ARETTQMTKS QILQQASTSV LAQAKQSPSA ALSLLG
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella. FlaB is not essential for flagellar synthesis and motility.
Gene References into Functions
  1. In both Sparus aurata and Oncorhynchus mykiss, the expression of genes encoding proinflammatory cytokines was induced upon stimulation of macrophages with the D1 domain synthetic flagellin from Vibrio anguillarum. PMID: 26363237
  2. 3 hours of stimulation with recombinant Flagellin B and its ND1 domain induced expression of the proinflammatory cytokines interleukin-1beta and tumor necrosis factor-alpha, and of the chemokine IL-8 in Sparus aurata and Oncorhynchus mykiss. PMID: 25449380
Subcellular Location
Secreted. Bacterial flagellum.
Protein Families
Bacterial flagellin family
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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