Recombinant Xenopus laevis Chordin (chrd), partial

Code CSB-YP005377XBE
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Source Yeast
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Code CSB-EP005377XBE
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Source E.coli
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Code CSB-EP005377XBE-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP005377XBE
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Source Baculovirus
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Code CSB-MP005377XBE
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
chrd
Uniprot No.
Alternative Names
chrdChordin; Organizer-specific secreted-dorsalizing factor
Species
Xenopus laevis (African clawed frog)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Potent dorsalizing factor. Has potent axis-forming activities including the ability to recruit neighboring cells into secondary axes. Regulates cell-cell interactions in the organizing centers of head, trunk and tail development.
Gene References into Functions
  1. Chordin protein formed a smooth gradient that encircled the embryo, reaching the ventral-most Brachet cleft. PMID: 24284174
  2. This study demonstrates a crucial role of Sizzled in coupling dorsal-ventral axis size information with Chordin stability. PMID: 23746842
  3. Bone morphogenetic protein 4 knockdown was sufficient to rescue the ventralizing effects caused by loss of Chordin activity. PMID: 15975940
  4. complex containing Smicl and the newly induced Xlim1 induces expression of Chordin PMID: 16192311
  5. Several lines of evidence support the hypothesis that chordin influences pronephros development by directing the formation of anterior somites. PMID: 17106888
  6. Embryo elongation can occur through the synergistic effect(s) of the organizer molecule chordin, and each of the 'verall posteriorizing molecules'eFGF, VegT and Xbra. PMID: 18318733
  7. Xld cleaves Chordin, an inhibitory binding protein for BMP2/4, releasing fragments with reduced affinity for these important ventralizing signals. PMID: 18664565
  8. CV2/Chordin interaction may help coordinate bone morphogenetic protein (BMP) diffusion to the ventral side of the embryo, ensuring that BMPs liberated from Chordin inhibition by tolloid proteolysis cause peak signaling levels. PMID: 18694564
  9. Study shows that Xenopus ONT1, an Olfactomedin-class secreted protein, stabilizes axial formation by restricting Chordin activity on the dorsal side. [ONT1] PMID: 18775317

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Subcellular Location
Secreted.
Protein Families
Chordin family
Tissue Specificity
First expressed at stage 9.5 in the dorsal marginal zone. Localized to the dorsal lip of the blastopore (Spemann organizer) during early gastrulation, after which expression continues in tissues derived from the organizer. Expressed in the prechordal plat
Database Links

KEGG: xla:398045

UniGene: Xl.3549

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