Recombinant Xenopus laevis Cysteine protease ATG4B (atg4b)

Code CSB-YP720642XBE
MSDS
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Source Yeast
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Code CSB-EP720642XBE
MSDS
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Source E.coli
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Code CSB-EP720642XBE-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP720642XBE
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP720642XBE
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
atg4b
Uniprot No.
Alternative Names
atg4b; apg4bCysteine protease ATG4B; EC 3.4.22.-; Autophagy-related protein 4 homolog B
Species
Xenopus laevis (African clawed frog)
Expression Region
1-384
Target Protein Sequence
MDAATLTYDT LRFADTPDFP ETAEPVWVLG RKYSALTEKE QLLNDITSRL WFTYRRNFQA IGGTGPTSDT GWGCMLRCGQ MIFAQALICR HVGRDWRWDK QKPKGEYLNI LTAFLDKKDS YYSIHQIAQM GVGEGKYIGQ WYGPNTVAQV LRKLAVFDQW SSIAVHIAMD NTVVVDEIRR LCRAGSGESS DAGALSNGYT GDSDPSCAQW KPLVLLIPLR LGLSEINEAY IETLKHCFMV PQSLGVIGGR PNSAHYFIGY VGDELIYLDP HTTQLSVEPS DCSFIEDESF HCQHPPCRMH VSEIDPSIAV GFFCSSQEDF EDWCQHIKKL SLSGGALPMF EVVDQLPLHL SNPDVLNLTP DSSDADRLDR FFDSEDEEFE ILSL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE). Has also an activity of delipidating enzyme for the PE-conjugated forms.
Subcellular Location
Cytoplasm.
Protein Families
Peptidase C54 family
Database Links

KEGG: xla:494717

UniGene: Xl.14362

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