Recombinant Xenopus laevis Glycogen synthase kinase-3 beta (gsk3b)

Code CSB-YP856250XBE
MSDS
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Source Yeast
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Code CSB-EP856250XBE
MSDS
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Source E.coli
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Code CSB-EP856250XBE-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP856250XBE
MSDS
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Source Baculovirus
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Code CSB-MP856250XBE
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
gsk3b
Uniprot No.
Alternative Names
gsk3b; Xgsk-3Glycogen synthase kinase-3 beta; GSK-3 beta; EC 2.7.11.26; Xgsk-3 protein
Species
Xenopus laevis (African clawed frog)
Expression Region
1-420
Target Protein Sequence
MSGRPRTTSF AESCKPVQQP SSFGSMKVSR DKDGSKVTTV VATPGQGPDR QQEVTYTDTK VIGNGSFGVV YQAKLCDTGE LVAIKKVLQD KRFKNRELQI MRKLDHCNIV RLRYFFYSSG EKKDEVYLNL VLDYVPETVY RVARHYSRAK QALPIIYVKL YMYQLFRSLA YIHSFGICHR DIKPQNLLLD PETAVLKLCD FGSAKQLVRG EPNVSYICSR YYRAPELIFG ATDYTSSIDV WSAGCVLAEL LLGQPIFPGD SGVDQLVEII KVLGTPTREQ IREMNPNYTE FKFPQIKAHP WTKVFRARTP PEAIALCSRL LEYTPTSRLT PLDACAHSFF DELRDPNLKL PNGREFPALF NFTTQELSSN PSLSSILIPA HARNQAAVST TSNTTSTSDS NTGERGSTNN AASASASNSS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Plays a role in the organization of the formation of the main body axis of developing embryo. Acts as an inhibitor of differentiation of primary neurons. Inhibits the ability of ectopically expressed NEUROD1 and other bHLH factors to promote early retinal cell differentiation. May participate in the Wnt signaling pathway. May regulate the circadian clock via phosphorylation of the major clock components.
Gene References into Functions
  1. These data suggest that the interactions of beta-catenin with alpha-catenin and GSK-3beta exert opposing effects on the terminal projections of ventral optic axons. PMID: 18425851
Subcellular Location
Cytoplasm. Nucleus. Cell membrane.
Protein Families
Protein kinase superfamily, CMGC Ser/Thr protein kinase family, GSK-3 subfamily
Database Links

KEGG: xla:399097

UniGene: Xl.324

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