Recombinant Xenopus laevis Mitogen-activated protein kinase 8 (mapk8)

Code CSB-YP013466XBE
MSDS
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Source Yeast
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Code CSB-EP013466XBE
MSDS
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Source E.coli
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Code CSB-EP013466XBE-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP013466XBE
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP013466XBE
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
mapk8
Uniprot No.
Alternative Names
mapk8; jnk1; Mitogen-activated protein kinase 8; MAP kinase 8; MAPK 8; EC 2.7.11.24; Stress-activated protein kinase JNK1
Species
Xenopus laevis (African clawed frog)
Expression Region
1-426
Target Protein Sequence
MSRSKRDSNF SVFEIGDSTF TVLKRYQNLK PIGSGAQGIV CAAFDAVLER HVAIKKLSRP FQNQTHAKRA YRELVLMKCV NHKNIIGLLN VFTPQKSLEE FQDLYIVMEL MDANLCQVIQ MELDHERMSY LLYQMLCGIK HLHSAGIIHR DLKPSNIVVK SDCTLKILDF GLARTAGTSF MMTPYVVTRY YRAPEVILGM GYKENVDIWS VGCILGEMIK GGVLFPGTDH IDQWNKVIEQ LGTPCTEFMK KLQPTVRTYV ENRPKYAGYS FEKLFPDVLF PADSEHNKLK ASQARDLLSK MLVIDASKRI SVDDALQHPY INVWYDPLEA EAPPPKIPDK QLDEREHTIE EWKELIYKEV LDWEERAKNG VIRGQPAPLG AAVTDGSQAH TSSSSGDASS MSTDPTLPSD TDSSLETSAG TLGCCR
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, and thus regulating transcriptional activity. Regulates morphogenic cell movements, controlling convergent extension during gastrulation. May play a role in the regulation of the circadian clock.
Gene References into Functions
  1. The results of this study suggest that JNK has a role in the disassembly adherens junctions by means of endocytosis that is required during buccopharyngeal membrane perforation. PMID: 28032936
  2. Hyperosmotic Shock Engages Two Positive Feedback Loops through Caspase-3-dependent Proteolysis of JNK1-2 and Bid. PMID: 26511318
  3. JNK signaling is required to establish microtubule stability and maintain tissue cohesion in the gut. PMID: 23462475
  4. Data show that the death pathway is independent of ERK but relies on activating Bad phosphorylation through the control of both kinases Cdk1 and JNK. PMID: 21858202
Subcellular Location
Cytoplasm. Nucleus. Cell junction, synapse.
Protein Families
Protein kinase superfamily, CMGC Ser/Thr protein kinase family, MAP kinase subfamily
Tissue Specificity
Strongly expressed in presumptive ectoderm and mesoderm regions and weakly expressed in endoderm regions during early stages of embryo development. Expressed in the head and dorsal regions during neurula and tailbud stages.
Database Links

KEGG: xla:379876

UniGene: Xl.8740

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