Recombinant Xenopus laevis DNA-binding protein inhibitor ID-3-A (id3-a)

Code CSB-YP841952XBE
MSDS
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Source Yeast
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Code CSB-EP841952XBE
MSDS
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Source E.coli
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Code CSB-EP841952XBE-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP841952XBE
MSDS
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Source Baculovirus
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Code CSB-MP841952XBE
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
id3-a
Uniprot No.
Alternative Names
id3-a; id3DNA-binding protein inhibitor ID-3-A; Inhibitor of DNA binding 3; XId3; XIdI; XIdIa; XIdIb; XIdx; Inhibitor of differentiation 3-A
Species
Xenopus laevis (African clawed frog)
Expression Region
1-118
Target Protein Sequence
MKAISPVRSM SSCYQAVCCL SEQSLSIARG SSHKGPGMDE PMGLLYDMNG CYSKLKELVP GIPQGSKLSQ VEILQHVIDY IFDLQIVLGE DQQQSSILSL QKSDFSELAT QGDTSVCH
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Influences cell fate decisions in the embryo by sequestering and blocking the activity of the bHLH transcription factors that control these decisions. Inhibits the binding of myogenic bHLH-containing complexes to E-box DNA, thereby preventing activation of muscle-specific target genes. Also inhibits the activity of neurogenic factor neurod1/neuroD. Plays a role in cell cycle progression and survival of neural crest progenitors; binding to either hes4-B/hairy2b or stat3 blocks the formation of transcription factor complexes and the repressor function of hes4-B/hairy2B, to allow neural crest progenitors to differentiate. May play a role in the regulation of the circadian rhythm.
Gene References into Functions
  1. Id3 functions by a novel mechanism, independent of cell fate determination, to mediate the decision of neural crest precursors to proliferate or die. PMID: 15769946
  2. Xenopus Id3 is required downstream of Myc for the formation of multipotent neural crest progenitor cells. PMID: 15772131
Subcellular Location
Nucleus.
Tissue Specificity
At gastrula stage, expressed in all three germ layers, but becomes localized to discrete domains of the developing nervous system during neurulation, including the anterior neural plate, cement gland, eye anlagen, otic placode and both cranial and trunk p
Database Links

KEGG: xla:379222

UniGene: Xl.57162

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