Recombinant Xenopus laevis ELAV-like protein 1-A (elavl1-a)

Code CSB-YP629266XBE
MSDS
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Source Yeast
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Code CSB-EP629266XBE
MSDS
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Source E.coli
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Code CSB-EP629266XBE-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP629266XBE
MSDS
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Source Baculovirus
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Code CSB-MP629266XBE
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
elavl1-a
Uniprot No.
Alternative Names
elavl1-a; elavl1; elrAELAV-like protein 1-A; 36 kDa embryonic-type cytoplasmic polyadenylation element-binding protein; 36 kDa eCPE-binding protein; 36 kDa eCPEB; p36; Protein ElrA-A; ElrA
Species
Xenopus laevis (African clawed frog)
Expression Region
1-337
Target Protein Sequence
MSNGYEDHMD DVCRDDIGRT NLIVNYLPQN MTQDELRSLF SSIGEVESAK LIRDKVAGFE MRSSSLSKGH SLGYGFVNYL NAKDAERAIN TLNGLRLQSK TIKVSFARPS SESIKDANLY ISGLPRTMTQ KDVEDMFLPF GHIINSRVLV DQATGLSRGV AFIRFDKRSE AEEAIASFNG HKPPGSSEPI TVKFAANPNQ NKNVALLSQI CHSPARRFGG PVHHQAQRFR FSPMGVDHMS SISSVNVASS ATSGWCIFVY NLGQDADEGI LWQMFGPFGA VTNVKVIRDF NTNKCKGFGF VTMTNYEEAA MAIASLNGYR LGDKTLQVSF KTSKSHK
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
RNA-binding protein that binds to the 3'-UTR region of mRNAs and increases their stability. Involved in embryonic stem cells (ESCs) differentiation: preferentially binds mRNAs that are not methylated by N6-methyladenosine (m6A), stabilizing them, promoting ESCs differentiation. Binds to poly-U elements and AU-rich elements (AREs) in the 3'-UTR of target mRNAs. May be involved in cytoplasmic mRNA polyadenylation. Acts cooperatively with cribp to stabilize AU-rich sequence (ARE)-containing mRNAs. May play a role during gastrulation. Required for the vegetal localization of vg1 mRNA.
Gene References into Functions
  1. ElrA functions in polyadenylation and stabilization of cyclin E1 mRNA. PMID: 17355986
Subcellular Location
Cytoplasm. Cytoplasm, cell cortex.
Protein Families
RRM elav family
Tissue Specificity
Ubiquitously expressed in adults.
Database Links

KEGG: xla:399292

UniGene: Xl.49136

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