Recombinant Xenopus laevis ELAV-like protein 2 (elavl2)

Code CSB-YP845937XBE
MSDS
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Source Yeast
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Code CSB-EP845937XBE
MSDS
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Source E.coli
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Code CSB-EP845937XBE-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP845937XBE
MSDS
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Source Baculovirus
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Code CSB-MP845937XBE
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
elavl2
Uniprot No.
Alternative Names
elavl2; el-1; elavl2-a; elrBELAV-like protein 2; Elav like-1; Xel-1; Protein ElrB; p45
Species
Xenopus laevis (African clawed frog)
Expression Region
1-389
Target Protein Sequence
MAVRLCDVAS LLRSGSWAAE PWTGQVIAAM ETQLSNGPTC NNTANCPNTI NCSSPVESNN TEDSKTNLIV NYLPQNMTQE ELKSLFGSIG EIESCKLVRD KITEGQSLGY GFVNYIDPKD AEKAINTLNG LRLQTKTIKV SYARPSSASI RDANLYVSGL PKTMTQKELE QLFSQYGRII TSRILVDQVT GVSRGVGFIR FDKRIEAEEA IKGLNGQKPP GATEPITVKF ANNPSQKVNH TILSQLYQSP NRRYPGPLAQ QAQRFRLDNL LNMAYGGIKS RFSPMAIDGM TSLAGINFPG HAGTGWCIFV YNLAPDADES ILWQMFGPFG AVTNVKVIRD FNTNKCKGFG FVTMTNYDEA AMAIASLNGY RLGDRVLQVS FKTSKTHKA
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Binds to poly-U elements and AU-rich elements (AREs) in the 3'-UTR of target mRNAs. Required for the vegetal localization of vg1 mRNA. Probably required for nervous system development.
Gene References into Functions
  1. elrB expression was not detected in Xenopus retina ganglion cell layer until stage 32. PMID: 15593335
  2. direct correlation between ElrB binding and translational repression by the Vg1 3'-UTR PMID: 16199879
  3. Data show that, while ElrA and ElrB are 69% identical and both exhibit RNA binding in the nM range, recombinant ElrB, but not ElrA, is able to oligomerise. PMID: 16930598
  4. Report on the identification of Xenopus ElrA/B proteins and show that Elr-type proteins are part of one RNP complex together with other localization proteins from Xenopus oocytes. PMID: 19458392
Subcellular Location
Cytoplasm. Cytoplasm, cell cortex.
Protein Families
RRM elav family
Tissue Specificity
Expressed in brain, testis and ovary. Ovarian expression is restricted to follicle cells surrounding the oocyte. From the early tailbud stage, expression is neural-specific and is seen in both the central and peripheral nervous system in differentiating n
Database Links

KEGG: xla:394342

UniGene: Xl.1648

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