Recombinant Xenopus laevis Protein snail homolog Sna (snai1)

Code CSB-YP322525XBE
MSDS
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Source Yeast
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Code CSB-EP322525XBE
MSDS
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Source E.coli
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Code CSB-EP322525XBE-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP322525XBE
MSDS
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Source Baculovirus
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Code CSB-MP322525XBE
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
snai1
Uniprot No.
Alternative Names
snai1; sna; Protein snail homolog Sna; Protein Xsnail; Protein xSna
Species
Xenopus laevis (African clawed frog)
Expression Region
1-259
Target Protein Sequence
MPRSFLVKKH FSASKKPNYS ELESQTVYIS PFIYDKFPVI PQPEILSTGA YYTPLVWDTG LLTTFFTSES DYKKSPISPS SSDDSSKPLD LTSFSSEDEG GKTSDPPSPA SSATEAEKFQ CNLCSKSYST FAGLSKHKQL HCDSQTRKSF SCKYCEKEYV SLGALKMHIR SHTLPCVCKI CGKAFSRPWL LQGHIRTHTG EKPFSCTHCN RAFADRSNLR AHLQTHSDVK KYQCKSCSRT FSRMSLLHKH EETGCTVAH
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcriptional repressor. Acts upstream of snai2/slug, zic5 and other neural crest markers in the specification of the neural crest and neural crest migration. Involved in embryonic mesoderm formation.
Gene References into Functions
  1. The transcription factor Snail1 is essential for tissue separation, enabling paraxial protocadherin (PAPC) to promote tissue separation through novel functions. PMID: 25778923
  2. the same E3 ubiquitin ligase known to regulate Snail family proteins, Partner of paired (Ppa), also controlled Twist stability and did so in a manner dependent on the Twist WR-rich domain PMID: 21727196
Subcellular Location
Nucleus.
Protein Families
Snail C2H2-type zinc-finger protein family
Tissue Specificity
Maternal expression is nearly completely restricted to the vegetal hemisphere. Zygotic expression begins in the dorsal marginal zone just before gastrulation (stage 9), and is almost completely absent in the animal hemisphere. At mid-gastrula (stage 11-11
Database Links

KEGG: xla:379615

UniGene: Xl.13309

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