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Recombinant Xenopus laevis Wee1-like protein kinase 2-A (wee2-a), partial

Recombinant Xenopus laevis Wee1-like protein kinase 2-A (wee2-a), partial

Code	CSB-YP342126XBE
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP342126XBE
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP342126XBE-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP342126XBE
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP342126XBE
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

wee2-a

Uniprot No.

P47817

Alternative Names

wee2-a; wee1a; Wee1-like protein kinase 2-A; EC 2.7.10.2; Maternally supplied wee1-like protein kinase 1A; Xe-wee1A

Species

Xenopus laevis (African clawed frog)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

■ Customer Reviews

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Target Background

Function

Oocyte-specific protein tyrosine kinase that phosphorylates and inhibits cdk1 and acts as a key regulator of meiosis. Required to maintain meiotic arrest in oocytes by phosphorylating cdk1 at 'Tyr-15', which inhibits cdk1 activity and prevents meiotic reentry. Negative regulator of mitosis. Involved in the mitotic DNA replication checkpoint.

Gene References into Functions

Cks2 regulates the phosphorylation of Wee1A by Cdk1 in a biphasic manner. PMID: 26854218
Consistent with regulating maternal mRNAs, Zar2 was present throughout oogenesis, and endogenous Zar2 co-immunoprecipitated endogenous Wee1 mRNA from immature oocytes, demonstrating the physiological significance of the protein-RNA interaction. PMID: 22732570
multisite phosphorylation cooperatively inactivates Wee1A and cooperatively promotes Wee1A proteolysis PMID: 16287869
Study found that the ultrasensitivity in the inactivation of Wee1 arises mainly through two competition mechanisms: competition between two sets of phosphorylation sites in Wee1 and between Wee1 and other high-affinity Cdk1 targets. PMID: 17382882
A novel mRNA 3' untranslated region translational control sequence regulates Xenopus Wee1 mRNA translation. PMID: 18395197

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Subcellular Location

Nucleus. Cytoplasm, cytosol.

Protein Families

Protein kinase superfamily, Ser/Thr protein kinase family, WEE1 subfamily

Tissue Specificity

Detected in egg (at protein level). Oocyte-specific maternally supplied protein. Present in immature and mature oocytes and in early (pregastrula) embryos, but not in post-gastrula embryos.

Database Links

KEGG: xla:398049

UniGene: Xl.1694

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