Recombinant Xenopus laevis Y-box-binding protein 2-A (ybx2-a)

Code CSB-YP321985XBE
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Source Yeast
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Code CSB-EP321985XBE
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Source E.coli
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Code CSB-EP321985XBE-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP321985XBE
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Source Baculovirus
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Code CSB-MP321985XBE
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ybx2-a
Uniprot No.
Alternative Names
ybx2-a; frgy2; frgy2-a; Y-box-binding protein 2-A; Cytoplasmic RNA-binding protein p56; Frog Y-box protein 2; FRG Y2; Frog Y-box protein 2-A; FRGY2a; Messenger ribonucleoprotein particle 4; mRNP4
Species
Xenopus laevis (African clawed frog)
Expression Region
1-336
Target Protein Sequence
MSEAEAQEPE PVPQPESEPE IQKPGIAAAR NQANKKVLAT QVQGTVKWFN VRNGYGFINR NDTKEDVFVH QTAIKKNNPR KFLRSVGDGE TVEFDVVEGE KGAEAANVTG PGGVPVKGSR FAPNRRRFRR RFYRPRADTA GESGGEGVSP EQMSEGERGE ETSPQQRPQR RRPPPFFYRR RFRRGPRPNN QQNQGAEVTE QSENKDPVAP TSEALASGDD PQRPPPRRFR QRFRRPFRPR PAPQQTPEGG DGETKAESGE DPRPEPQRQR NRPYVQRRRR QGATQVAATA QGEGKAEPTQ HPASEEGTPS DSPTDDGAPV QSSAPDPGIA DTPAPE
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Has a dual function in oocytes: as a DNA-binding protein, binds to the Y-box sequence (CTGATTGGCCAA) in the promoters of target genes to stimulate transcription. May also function at CCAAT-containing promoters that lack the consensus Y-box sequence. Also binds mRNA to promote accumulation of the transcripts it contributes to produce.
Gene References into Functions
  1. findings showed co-localization of Xtr with FRGY2 in germ plasm in which XDead end mRNA has been shown to be localized specifically; proposed the possible role of Xtr in translational activation of the maternal mRNAs repressed in mRNP particle PMID: 21314676
  2. FRGY2a and YB1 disassemble nucleoli by sequestering B23, which is associated with pre-ribosomes and other structurally important nucleolar components PMID: 16415342
Subcellular Location
Cytoplasm. Note=Either free or associated with ribonucleoprotein particles.
Tissue Specificity
In adults, expression is restricted to the ovary and testis.
Database Links

KEGG: xla:397746

UniGene: Xl.787

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