Recombinant Xenopus tropicalis Iroquois-class homeodomain protein irx-3(irx3)

Code CSB-YP744000XBF
Size Pls inquire other sizes
Source Yeast
Code CSB-EP744000XBF
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Source E.coli
Code CSB-EP744000XBF-B
Size Pls inquire other sizes
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Code CSB-BP744000XBF
Size Pls inquire other sizes
Source Baculovirus
Code CSB-MP744000XBF
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Source Mammalian cell

Product Details

Purity >85% (SDS-PAGE)
Target Names irx3
Uniprot No. Q6NVN3
Species Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Expression Region 1-448
Target Protein Sequence MSFPQLGYQY IRPLYPSDRQ NVGGTRSGTE LSPAGTLSNV LSSVYGTPYA AAAAAQAYGA FLPYSAELPI FPQLGSQYEM KDSPGVQHAA FSHPHAAFYP YGQYQFGDPS RPKNATREST STLKAWLNEH RKNPYPTKGE KIMLAIITKM TLTQVSTWFA NARRRLKKEN KMTWAPRSRT DEEGNAYGSD HEEDKHEDDE EIDLENIDTE DIESKEDLDD PDTDIHSDSK TDARSDSEAS DGFEDLNAPE DRLLKSVVGQ RQVLNEEPQD KCALSSDAKA SQPACEQIKL DRIPSSPPLE NNIPAAHKPK IWSLAETATT PDNPRRSPNT GGSVNTQNLI AQHRLIASPG SRFQGWTGRA FSAQQLSLLN SAHFLQGLSV SHTALGSGTA SFPKAAEPKH STDSLTDRSS TVDIEKKIPV LNTAFQPVQR RSQNQLDAAM ILSALSSS
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.
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Target Data

Function Acts partially redundantly with other irx members in neural patterning. Required for formation of the posterior forebrain, midbrain, hindbrain, and to a lesser extent, spinal cord. Both up-regulates and down-regulates gene expression during neural development. Acts early in neural plate development to induce proneural gene expression and specify a neural precursor state. Also up-regulates repressors that prevent neuronal differentiation. Required during at least two stages of pronephros kidney development; during neurula stages, maintains transcription of key renal genes to define the size and identity of the pronephric anlage, probably in part through regulation of bmp-signaling. Subsequently required for proper formation of the intermediate tubule segment of the pronephros.
Subcellular Location Nucleus
Protein Families TALE/IRO homeobox family
Tissue Specificity Expressed in the neural plate in overlapping patterns with other irx members, which all share an anterior border of expression. Outside the nervous system and at tailbud stages, expressed in the developing otic vesicle, branchial arches, prospective heart
Database Links

KEGG: xtr:407888

STRING: 8364.ENSXETP00000012797

UniGene: Str.51982

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