Recombinant Xenopus tropicalis Iroquois-class homeodomain protein irx-3 (irx3)

Code CSB-YP744000XBF
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Source Yeast
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Code CSB-EP744000XBF
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Source E.coli
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Code CSB-EP744000XBF-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP744000XBF
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Source Baculovirus
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Code CSB-MP744000XBF
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
irx3
Uniprot No.
Alternative Names
irx3; TNeu131p22.1; Iroquois-class homeodomain protein irx-3; Iroquois homeobox protein 3
Species
Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Expression Region
1-448
Target Protein Sequence
MSFPQLGYQY IRPLYPSDRQ NVGGTRSGTE LSPAGTLSNV LSSVYGTPYA AAAAAQAYGA FLPYSAELPI FPQLGSQYEM KDSPGVQHAA FSHPHAAFYP YGQYQFGDPS RPKNATREST STLKAWLNEH RKNPYPTKGE KIMLAIITKM TLTQVSTWFA NARRRLKKEN KMTWAPRSRT DEEGNAYGSD HEEDKHEDDE EIDLENIDTE DIESKEDLDD PDTDIHSDSK TDARSDSEAS DGFEDLNAPE DRLLKSVVGQ RQVLNEEPQD KCALSSDAKA SQPACEQIKL DRIPSSPPLE NNIPAAHKPK IWSLAETATT PDNPRRSPNT GGSVNTQNLI AQHRLIASPG SRFQGWTGRA FSAQQLSLLN SAHFLQGLSV SHTALGSGTA SFPKAAEPKH STDSLTDRSS TVDIEKKIPV LNTAFQPVQR RSQNQLDAAM ILSALSSS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Acts partially redundantly with other irx members in neural patterning. Required for formation of the posterior forebrain, midbrain, hindbrain, and to a lesser extent, spinal cord. Both up-regulates and down-regulates gene expression during neural development. Acts early in neural plate development to induce proneural gene expression and specify a neural precursor state. Also up-regulates repressors that prevent neuronal differentiation. Required during at least two stages of pronephros kidney development; during neurula stages, maintains transcription of key renal genes to define the size and identity of the pronephric anlage, probably in part through regulation of bmp-signaling. Subsequently required for proper formation of the intermediate tubule segment of the pronephros.
Subcellular Location
Nucleus.
Protein Families
TALE/IRO homeobox family
Tissue Specificity
Expressed in the neural plate in overlapping patterns with other irx members, which all share an anterior border of expression. Outside the nervous system and at tailbud stages, expressed in the developing otic vesicle, branchial arches, prospective heart
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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