Recombinant Yersinia pestis bv. Antiqua Anhydro-N-acetylmuramic acid kinase (anmK)

Code CSB-YP625479YAG
MSDS
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Source Yeast
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Code CSB-EP625479YAG
MSDS
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Source E.coli
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Code CSB-EP625479YAG-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP625479YAG
MSDS
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Source Baculovirus
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Code CSB-MP625479YAG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
anmK
Uniprot No.
Alternative Names
anmK; YPN_1829; YP516_2031Anhydro-N-acetylmuramic acid kinase; EC 2.7.1.170; AnhMurNAc kinase
Species
Yersinia pestis bv. Antiqua (strain Nepal516)
Expression Region
1-370
Target Protein Sequence
MKSGRFIGVM SGTSLDGVDV VLAAIDERMV AQQASYTHPI PLQLKKDILG MCQGQSTTLS AVGKLDAQLG ILFAEAVLAL LAKEGLSAQD ITAIGCHGQT VWHEPLGEPA FTMQLGDNNR IAAMTQIATV GDFRRRDMAY GGQGAPLVPA FHHALLAHAT EKRMVLNIGG IANLSVLLPD SPIRGFDTGP GNMLMDAWIW RNCSLPYDKD ACWALSGHVN QPLLEQMFND PYFRLPAPKS TGREYFNAAW LDKQLARIPG VTAEDIQATL AELTAVSITE QVRLAGGCDR LLVCGGGARN PLVMARISAL LSGTEVCTTD DAGIRGDDME ALAFAWLAFR TLSGKPGNLP SVTGASRETI LGAVHPVSSW
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Catalyzes the specific phosphorylation of 1,6-anhydro-N-acetylmuramic acid (anhMurNAc) with the simultaneous cleavage of the 1,6-anhydro ring, generating MurNAc-6-P. Is required for the utilization of anhMurNAc either imported from the medium or derived from its own cell wall murein, and thus plays a role in cell wall recycling.
Protein Families
Anhydro-N-acetylmuramic acid kinase family
Database Links

KEGG: ypn:YPN_1829

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