Code | CSB-RA010418A14acHU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:2000 |
ICC | 1:50-1:500 |
IF | 1:30-1:200 |
The development of the acetyl-Histone H3.1 (K14) recombinant monoclonal antibody begins with the cloning of genes responsible for encoding the HIST1H3A antibody, encompassing both heavy and light chains. These cloned genes are then inserted into an expression vector, which is subsequently introduced into host cells through transfection. The host cells are cultured to produce and secrete the antibody. Following this, the antibody undergoes purification using affinity chromatography to ensure its purity and effectiveness. Rigorous testing then confirms its functionality in various applications, including ELISA, WB, ICC, and IF, allowing for precise detection of the human and rat
HIST1H3A proteins acetylated at K14.
Acetylation of histone H3.1 at lysine 14 (K14) primarily functions in transcriptional activation, chromatin accessibility, cellular identity, epigenetic memory, and coordinated gene regulation, and has implications in various diseases.
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