Code | CSB-RA010418A09me2HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:5000 |
IF | 1:30-1:200 |
To manufacture the di-methyl-histone H3.1 (K9) recombinant monoclonal antibody, the journey begins with the retrieval of genes responsible for encoding the HIST1H3A antibody from rabbits that have previously undergone immunization with a synthesized peptide originating from the human HIST1H3A protein di-methylated at K9. Subsequently, these antibody genes are seamlessly integrated into specialized expression vectors. Following this genetic modification, the vectors are thoughtfully introduced into host suspension cells, which are diligently cultivated to encourage the production and secretion of antibodies. After this growth phase, the di-methyl-histone H3.1 (K9) recombinant monoclonal antibody undergoes a meticulous purification process using affinity chromatography techniques, effectively isolating the antibody from the surrounding cell culture supernatant. Lastly, the functionality of the antibody is rigorously assessed through a battery of tests, including ELISA, WB, and IF, conclusively affirming its ability to effectively react with the human HIST1H3A protein di-methylated at K9.
Di-methylated K9 HIST1H3A is also found at centromeres, which are essential for proper chromosome segregation during cell division. When HIST1H3A is di-methylated at K9, it contributes to the repression of gene transcription. This modification creates a repressive chromatin environment by preventing the binding of transcription factors and other regulatory proteins to the DNA.
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