Product Details

Uniprot No.

Target Names

HIST2H2BE

Alternative Names

HIST2H2BE antibody; H2BFQHistone H2B type 2-E antibody; Histone H2B-GL105 antibody; Histone H2B.q antibody; H2B/q antibody

Species Reactivity

Human, Mouse

Immunogen

A synthesized peptide

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

3E12

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

To manufacture the mono-methyl-histone H2B type 2-E (R79) recombinant monoclonal antibody, the journey begins with the retrieval of genes responsible for encoding the HIST2H2BE antibody from rabbits that have previously undergone immunization with a synthesized peptide originating from the human HIST2H2BE protein mono-methylated at R79. Subsequently, these antibody genes are seamlessly integrated into specialized expression vectors. Following this genetic modification, the vectors are thoughtfully introduced into host suspension cells, which are diligently cultivated to encourage the production and secretion of antibodies. After this growth phase, the mono-methyl-histone H2B type 2-E (R79) recombinant monoclonal antibody undergoes a meticulous purification process using affinity chromatography techniques, effectively isolating the antibody from the surrounding cell culture supernatant. Lastly, the functionality of the antibody is rigorously assessed through ELISA, conclusively affirming its ability to effectively react with the human HIST2H2BE protein mono-methylated at R79.

Target Background

Function

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.; Has broad antibacterial activity. May contribute to the formation of the functional antimicrobial barrier of the colonic epithelium, and to the bactericidal activity of amniotic fluid.

Gene References into Functions

Findings uncover a novel function of let-7 miRNAs as regulators of H2B ubiquitylation, suggesting an additional mechanism whereby these miRNAs can exert their tumor-suppressive effects. PMID: 28604753
Within the nucleus, PKC-theta; catalytic activity maintains the Ser536 phosphorylation on the p65 subunit of NF-kappaB (also known as RelA) and can directly influence chromatin accessibility at transcriptional memory genes by regulating H2B deposition. PMID: 27149922
Study provides the first observation of the proteolytic processing of histones H2B and H3 in human hepatocytes where four different clipping sites were localized in H3 and one in H2B. PMID: 26424599
Gene expression analysis identified deregulation of histone H2A and H2B genes in all four cell lines ;histone pathways are associated with epirubicin resistance PMID: 26852132
The 2.7-A-resolution crystal structure of the human YL1-H2A.Z-H2B complex shows that YL1 binding, similarly to ANP32E binding, triggers an extension of the H2A.Z alphaC helix. PMID: 26974126
CRL4(Wdr70) regulates H2B monoubiquitination and facilitates Exo1-dependent DNA repair resection. PMID: 27098497
analysis of a functional hotspot on ubiquitin that is required for the stimulation of human Dot1 PMID: 26240340
Results show that the cotranscriptional ubiquitylation of histone H2B suggests that it may be more intimately involved in ongoing nucleosome turnover in the wake of Pol II. PMID: 25049226
H2B K34 ubiquitylation by the MOF-MSL complex is part of the protein networks involved in early steps of transcription elongation. PMID: 24837678
Knockdown of EZH2 increased the ubiquitination level of H2BK120. PMID: 24339737
Autoimmunity to isoAsp histone H2B suggests that this form of the autoantigen may be critical in the induction of anti-histone autoantibodies in human SLE. PMID: 22967069
after H2B monoubiquitylation H2B is largely positioned at the exon-intron boundaries of highly expressed exons, and it demonstrates increased occupancy in skipped exons compared with flanking exons in the human genome PMID: 22421545
cancerous cells in the tumor specimens. Taken together, our data suggest that glucose deficiency and loss of uH2B are novel properties of cancer cells in vivo, which may represent important regulatory mechanisms of tumorigenesis PMID: 22615809
H2B and H4 histones were mobilized during herpes simplex virus 1 infection and became available to bind to viral genomes. PMID: 21994445
Studies indicate that H2B monoubiquitylation is driven primarily by an E3 ubiquitin ligase composed of the two RING finger proteins RNF20 and RNF40. PMID: 21827756
these data point at acetylation of Lysine 120 of H2B as an early mark of poised or active state and establish a temporal sequence between acetylation and mono-ubiquitination of this H2B residue. PMID: 21739721
Protein phosphatase 2C gamma binds to/dephosporylates histone 2b to affect chromatin function. PMID: 17074886
A redox-modulated direct p38/GAPDH-Oct-1 interaction nucleates the occupancy of the H2B promoter by the OCA-S complex, in which p36/LDH plays a critical role in the hierarchical organization of the complex. PMID: 18682386
These studies establish the natural H2B ubiquitylation factors in human cells and also detail the mechanistic basis for H2B ubiquitylation and function during transcription PMID: 19410543
NO-mediated H2B gene repression depends on modifications of endogenous PPAR ligands. PMID: 19425504