Code | CSB-RA010418A18me1HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:2000 |
ICC | 1:50-1:500 |
IF | 1:30-1:200 |
In the development of the mono-methyl-histone H3.1 (K18) recombinant monoclonal antibody, the initial phase comprises the retrieval of genes responsible for coding the HIST1H3A antibody. These genes are acquired from rabbits that have been previously exposed to a synthesized peptide derived from the human HIST1H3A protein mono-methylated at K18. Subsequently, these antibody genes are seamlessly integrated into specialized expression vectors. Following this genetic modification, the vectors are introduced into host suspension cells, which are carefully cultured to stimulate the expression and secretion of antibodies. Following this cultivation phase, the mono-methyl-histone H3.1 (K18) recombinant monoclonal antibody is subjected to a thorough purification process utilizing affinity chromatography techniques, effectively separating the antibody from the surrounding cell culture supernatant. Ultimately, the functionality of the antibody is comprehensively evaluated through a diverse array of assays, including ELISA, WB, ICC, and IF tests, unequivocally confirming its capacity to interact with the human HIST1H3A protein mono-methylated at K18.
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