Code | CSB-RA010418A36me1HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:2000 |
ICC | 1:50-1:500 |
IF | 1:30-1:200 |
The production of the mono-methyl-histone H3.1 (K36) recombinant monoclonal antibody comprises the cloning of genes responsible for encoding the HIST1H3A antibody and their subsequent expression within mammalian cell expression systems. This process involves the cloning of both heavy and light chain genes of the HIST1H3A antibody into expression vectors, which are later introduced into host cells through transfection. Subsequently, the host cells take on the role of producing and secreting the antibodies. Following production, the antibody undergoes purification using affinity chromatography techniques. The final antibody product is rigorously tested for functionality across a range of applications, including ELISA, WB, ICC, and IF, specifically designed for the accurate detection of the human HIST1H3A protein mono-methylated at K36.
Mono-methylation of HIST1H3A at lysine 36 (K36) primarily functions in transcriptional repression and chromatin compaction, with roles in alternative splicing, DNA repair, cellular identity, epigenetic memory, and implications in disease. It is a crucial epigenetic modification that helps regulate gene expression and chromatin structure.
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