Code | CSB-RA010418A14meHU |
Size | US$210 |
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The process of generating the mono/di/tri-methyl-histone H3.1 (K14) recombinant antibody starts with the cloning of genes encoding the HIST1H3A antibody, encompassing both heavy and light chains. These cloned genes are then incorporated into expression vectors, which are introduced into host cells via transfection. The host cells are responsible for producing and secreting the antibody. Following purification using affinity chromatography to ensure its purity, the antibody undergoes stringent functionality testing through ELISA, facilitating accurate detection of the human HIST1H3A protein mono/di/tri-methylated at K14.
H3.1 mono-methylation at K14 is associated with both transcriptional activation and repression, depending on the context. Di- or tri-methylation at H3.1 K14 is often linked to transcriptional repression. Di-methylation at H3.1 K14 can be involved in the regulation of genes associated with development, differentiation, and cell cycle control. Tri-methylation at H3.1 K14 is involved in the regulation of genes important for cellular identity, development, and genome stability.
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