Code | CSB-RA010418A79meHU |
Size | US$210 |
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To create the mono/di/tri-methyl-histone H3.1 (K79) recombinant monoclonal antibody, the process initiates with the isolation of genes responsible for coding the HIST1H3A antibody from rabbits that have been previously exposed to a synthesized peptide originating from the human HIST1H3A protein methylated at K79. These antibody genes are then meticulously integrated into specialized expression vectors. Following this genetic modification, the vectors are thoughtfully introduced into host suspension cells, which are diligently cultivated to encourage the production and secretion of antibodies. After this cultivation phase, the mono/di/tri-methyl-histone H3.1 (K79) recombinant monoclonal antibody undergoes a rigorous purification process using affinity chromatography techniques, effectively separating the antibody from the surrounding cell culture supernatant. Finally, the functionality of the antibody is comprehensively assessed through ELISA, conclusively confirming its capability to interact effectively with the human HIST1H3A protein methylated at K79.
K79 methylation on HIST1H3A is often found at silenced or inactive gene loci. It marks these chromatin regions as transcriptionally inactive, preventing the binding of transcription factors and other regulatory proteins to the DNA. This leads to the repression of nearby genes. It is involved in proper chromosome organization and segregation during cell division.
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