Code | CSB-RA010418A10phHU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:2000 |
ICC | 1:50-1:500 |
IF | 1:30-1:200 |
To create the phospho-histone H3.1 (S1) recombinant monoclonal antibody, genes encoding the HIST1H3A antibody are first cloned, encompassing both heavy and light chains. These cloned genes are then inserted into expression vectors, which are introduced into host cells via transfection. The host cells are responsible for the production and secretion of the antibody. Affinity chromatography is employed to ensure the antibody's purity, after which it undergoes rigorous functionality testing in various applications, including ELISA, WB, ICC, and IF, enabling accurate detection of the human HIST1H3A protein phosphorylated at S1.
Phosphorylation at H3.1 S1 can promote chromatin condensation thus leading to gene repression. During mitosis, histone H3.1 S1 phosphorylation plays a role in chromosome condensation and segregation. It helps ensure accurate cell division by regulating chromosomal architecture. Phosphorylated H3.1 S1 is also associated with DNA damage response and repair processes. H3.1 S1 phosphorylation is also linked to cell cycle regulation, epigenetic signaling, and coordinated gene regulation.
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