SLC25A12 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA234248A0HU
Size	US$210
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Image	Flow cytometric analysis of solute carrier family 25 member 12 expression in C2C12 cells using solute carrier family 25 member 12 antibody. Green, isotype control; red, solute carrier family 25 member 12. Immunocytochemical staining of C2C12 cells with solute carrier family 25 member 12 antibody. Nuclei were stained blue with DAPI; Solute carrier family 25 member 12 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm. Western blotting analysis using solute carrier family 25 member 12 antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with solute carrier family 25 member 12 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.
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Product Details
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Target Background

Product Details

Uniprot No.

O75746

Target Names

SLC25A12

Alternative Names

SLC25A12; Solute Carrier Family 25 Member 12; Aralar; AGC1; Electrogenic Aspartate/Glutamate Antiporter SLC25A12, Mitochondrial; Aralar1; Solute Carrier Family 25 (Mitochondrial Carrier, Aralar), Member 12; Solute Carrier Family 25 (Aspartate/Glutamate Carrier), Member 12; Mitochondrial Aspartate Glutamate Carrier 1; Aspartate/Glutamate Carrier 1; Araceli Hiperlarga; Calcium Binding Mitochondrial Carrier Superfamily Member Aralar1; Calcium-Binding Mitochondrial Carrier Protein Aralar1; ARALAR1; EIEE39; DEE39

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant Human SLC25A12 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

23C3

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.

Form

Liquid

Tested Applications

ELISA, WB, FC, ICC

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
FC	1:200-1:2000
ICC	1:100-1:1000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Mitochondrial and calcium-binding carrier that catalyzes the calcium-dependent exchange of cytoplasmic glutamate with mitochondrial aspartate across the mitochondrial inner membrane. May have a function in the urea cycle.

Gene References into Functions

Genetic variants of SLC25A12 may be associated with risks for childhood ASD. PMID: 28536923
The features of AGC1 structure and function in physiology and pathology, regulation by calcium, dependency on mitochondrial membrane potential, role in cancer cells, and tissue specificity are reviewed. AGC1 is involved in the glutamate-mediated excitotoxicity in neurons and AGC gene or protein alterations were discovered in rare human diseases. Review. PMID: 27132995
Sensitivity analyses including only studies with family-based design demonstrated significant association between autism spectrum disorders and SNPs rs2292813 and rs2056202. In contrast, sensitivity analyses including case-control design studies only failed to find a significant association. Review. PMID: 25663199
rs2056202 and rs2292813 in SLC25A12 may contribute significantly to autism spectrum disorders risk. PMID: 25921325
Structure of the calcium bound and calcium free N- and C-terminal domains is described, elucidating the mechanism of calcium regulation. PMID: 25410934
The physiological roles of AGC1, its links to calcium homeostasis, and its involvement in autism pathogenesis, are reviewed. PMID: 21691713
This study found no differences in the allele, genotype, or haplotype frequencies of these two SNPs between patients and controls. PMID: 19913066
Variants of the AGC1-encoding SLC25A12 gene were neither correlated with AGC activation nor associated with autism-spectrum disorders in 309 simplex and 17 multiplex families. PMID: 18607376
SLC25A12 gene is linked to autism PMID: 15056512
Aralar1 has a role in determining glucose metabolic fate, mitochondrial activity, and insulin secretion in beta cells PMID: 15494407
These results suggest that SLC25A12 is not a major contributor to autism risk in these families. PMID: 16648338
it is unlikely that the SLC25A12 polymorphisms investigated play a substantial role in conferring susceptibility to schizophrenia PMID: 17693006
rs2056202 polymorphism in SLC25A12 may be associated with levels of routines and rituals in autism and related disorders PMID: 17894412
SLC25A12 expression is associated with neurite outgrowth and is upregulated in the prefrontal cortex of autistic subjects. PMID: 18180767
SLC25A12 gene is associated with autism. PMID: 19360665

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Involvement in disease

Epileptic encephalopathy, early infantile, 39 (EIEE39)

Subcellular Location

Mitochondrion inner membrane; Multi-pass membrane protein.

Protein Families

Mitochondrial carrier (TC 2.A.29) family

Tissue Specificity

Expressed predominantly in the heart and skeletal muscle, weakly in brain and kidney.

Database Links

HGNC: 10982

OMIM: 603667

KEGG: hsa:8604

STRING: 9606.ENSP00000388658

UniGene: Hs.470608

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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