Fast Pfu DNA Polymerase

Code CSB005B
Size 1000 Units
Relevance Pfu DNA Polymerase is a fast and high fidelity DNA polymerase, This enzyme is made by the fusion of a special protein based on common Pfu DNA polymerase through the protein molecular modification technology. It guarantees the high fidelity and at the same time the extending speed is upgraded to 3-4kb/min in PCR process,which is 4-8 times that of ordinary Pfu DNA polymerase. It does not exhibit nucleotidyl terminal transferase activity so its amplification products can be directly used for cloning in blunt-ended vectors.
Intended Use 1 DNA amplification by Polyermerase Chain Reaction (PCR). 2 High-fidelity DNA amplification, cloning and expression 3 DNA sequencing. 4 Site directed mutagenesis 5 Gene synthesis.
Quality Control The purity of enzyme is above 97%, evaluated by SDS-PAGE. It’s validated to be no exogenous nucleic acid enzymatic activity. There is no residual host cell DNA by PCR detection. Functionally tested in amplification of a single-copy gene from human genomic DNA. No obvious enzyme activity changes observed after storing at room temperature for one week.
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Storage -20°C
Length/Coupling Ratio 2.5U/μl
Fast Pfu DNA Polymerase 400ul
10×Pfu buffer (Mg2+Plus) 1ml×4
dNTP Mixture (10mM each) 200μl×4
6×loading buffer 1ml×4

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