Cusa Taq DNA Polymerase

Code CSB002A
Size 250 Units
Relevance

CusaTaq DNA Polymerase is a recombinant 94 kDa DNA polymerase (deoxynucleoside-triphosphate: DNA deoxynucleotidyltransferase, EC 2.7.7.7), originally inducibly expressed in and purified from E.coli. that containing DNA polymerase I, thermostable gene. It has the same function as native Taq DNA polymerase. The enzyme has a 5 ' -3 ' DNA polymerase activity and 5 ' -3 ' exonuclease activity, but without 3 ' -5 ' exonuclease activity. The extend speed of CusaTaq DNA polymerase is 1-2kb/min in PCR process and the final product could be directly cloned into T-Vector since it has an “A” at the 3’ end.

Intended Use 1 DNA amplification by Polyermerase Chain Reaction (PCR). 2 DNA sequencing. 3 DNA labeling. 4 Primer extension. 5 Single 3´-thymidine (T) overhangs for TA Cloning.
Quality Control The purity of enzyme is above 97%, evaluated by SDS-PAGE. It’s validated to be no exogenous nucleic acid enzymatic activity. Taking human genome DNA as template, the enzyme effectively amplifies 3.0kb DNA fragment. No obvious enzyme activity changes observed after storing at room temperature for one week.
Storage -20°C
Length/Coupling Ratio 5U/μl
Component
Cusa Taq DNA Polymerase 50ul
10×PCR buffer(Mg2+ Plus) 1ml
dNTP Mixture(10mM each) 100μl
6×loading buffer 1ml
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