Taq DNA Polymerase

Specification

Code

CSB002B

Size

1000 Units

Relevance

Taq Hot Start DNA Polymerase has a 5 ' -3 ' DNA polymerase activity and 5 ' -3 ' exonuclease activity, but without 3 ' -5 ' exonuclease activity, that containing anti-Taq antibody and Taq. This enzyme is suitable for Hot Start PCR, and can effectively restrain the formation of primer dimers and other non-specific amplification. Anti-Taq antibody in the pre degeneration stage of PCR becomes inactive,without special treatment. The extend speed of Taq DNA polymerase is 1-2kb/min in PCR process and the final product could be directly cloned into T-Vector since it has an “A” at the 3’ end.

Intended Use

1 DNA amplification by Polyermerase Chain Reaction (PCR). 2 DNA sequencing. 3 DNA labeling. 4 Primer extension. 5 Single 3´-thymidine (T) overhangs for TA Cloning.

Quality Control

The purity of enzyme is above 97%, evaluated by SDS-PAGE. It’s validated to be no exogenous nucleic acid enzymatic activity. Taking human genome DNA as template, the enzyme effectively amplifies 3.0kb DNA fragment. No obvious enzyme activity changes observed after storing at room temperature for one week.

Storage

-20°C

Length/Coupling Ratio

5U/μl

Component

Taq DNA Polymerase	200ul
10×PCR buffer (Mg²⁺ Plus)	1ml×4
dNTP Mixture (10mM each)	100μl×4
6×loading buffer	1ml×4

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Taq DNA Polymerase

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