Recombinant Bacillus subtilis Stage III sporulation protein AE (spoIIIAE)

Code CSB-CF342248BRJ
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Source in vitro E.coli expression system
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Product Details

Target Names
spoIIIAE
Uniprot No.
Alternative Names
spoIIIAE; BSU24390; Stage III sporulation protein AE
Species
Bacillus subtilis (strain 168)
Expression Region
25-399
Target Protein Sequence
AGNAEQTEDHAETAEQLAERTAASLETDKIGEFWNDIMTEYGGLLPESQKGSLMEFINGD KSFSPQEWLKALFSYLFHEVLANGKLLGTLILLTIFCVILQLLQNAFQQSTVSKVAYSIV YMVLIILALNSFHVAINYATEAIQTMTSFILALIPLLLALLASSGGAVSAAFFHPVILFL MNTSGLLIQNIVMPLIFLSAILSIVSTMTEQYKVTQLANLLRNIAIGALAVFLTIFLGVI SVQGASAAVTDGITLRTAKFITGNFIPVLGRMFTDATDTVISASLLLKNTVGILGVAILI CIAAFPAIKVLSLAFIYKLAAAILQPLGGGPVITCLDVISKSVIYIFAALAIVSLMFFLS LTVIITAGNLTMMMK
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Required during sporulation for activation of sigma factor SpoIIIG/SigG after engulfment is completed in the prespore. Overexpression in the absence of SpoIIIJ is synthetically lethal.
Subcellular Location
Cell membrane; Multi-pass membrane protein. Note=Signal sequence cleavage facilitates function; replacing the signal with the first transmembrane region of SpoIVFB decreases sporulation 7-fold, whereas replacing it with the SleB signal sequence does not affect sporulation.
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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