Recombinant Cowpea severe mosaic virus RNA1 polyprotein

Code CSB-CF334116CRE
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Source in vitro E.coli expression system
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Product Details

Uniprot No.
Alternative Names
RNA1 polyprotein; Genome polyprotein B; P1
Species
Cowpea severe mosaic virus (strain DG) (CPSMV)
Expression Region
1147-1858
Target Protein Sequence
AAEKFFDFLEKEQHVTEGIGKVGNLKKGVWVPLPTKTNLVETPKEWHLGTEKTKEPSILS STDLRLGDKQYDPFVGGIQKYAEPMGILDDEVLRHVATDIVEEWFDCVDPQEDTFEEVDL QVAINGLEGMEYMERVPMATSEGFPHILTRKSGEKGKGRFVYGDGEIFDLIPGTSVHEAY LTLEETCADTVPALVGIECPKDEKLPLRKIYEKPKTRCFTVLPMEYNLVVRRKFLKFVVF IMKNRHRLSCQVGINPYGMEWSRLAMSLLEKGNNILCCDYSSFDGLLTKQVMHLMSEMIN ELCGGSSRLKQQRTNLLMACCSRYALCKGEVWRVECGIPSGFPLTVICNSIFNELLVRYS YIKICQQARVPATITYGFSTFVKMVTYGDDNLLSVQSAITHVFDGTKLKEFLKLNGITIT DGKDKTSPVLNFRNLEDCDFLKRGFKKESDVVWVGPEEKESLWAQLHYVTTNNLEKHEAY LVNVVNVIRELYLHDPREAAELRRKAIQNVDFLKENPKDLPTMAAIKEFYNMQRQQQFVD SNDNLDSLLNPDFLFVAPHRKMHEAEMELVPKWYLRDLGKAPINVLTGEADRICVLVNAS IPDHLLPEKVVNISWPYGPGRGGLPTHGWAQANLYNPNSAVVKKLRTLVNQNPDDRVDIC FRHDAVPVAIATIIFLVHLGKVKGRSANEYLTKIIDSAKSLKFLPKECDIIF
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Thiol protease that cleaves the RNA1 and RNA2 polyproteins.; Plays a role in RNA replication. It is covalently linked to the 5'terminus of both viral single-stranded RNA1 and RNA2 molecules.; Down-regulates the RNA1 polyprotein processing and enhances trans-cleavage of RNA2 polyproteins. The protease cofactor and the putative helicase seem to target the replication complexes to ER membranes. Their physical association causes the membrane rearrangement of host ER that may result in formation of the small membranous vesicles that are the site of viral RNA synthesis.; The protease cofactor and the putative helicase seem to target the replication complexes to ER membranes. Their physical association causes the membrane rearrangement of host ER that may result in formation of the small membranous vesicles that are the site of viral RNA synthesis.; Replicates the viral genome.
Subcellular Location
[Putative helicase]: Host membrane; Single-pass membrane protein. Host cytoplasm, host perinuclear region.; [RNA-directed RNA polymerase]: Host endoplasmic reticulum.; [Protease cofactor]: Host cytoplasm, host perinuclear region.
Protein Families
Comoviridae genome polyprotein B family
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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