Recombinant Dog Triadin (TRDN)

Code CSB-CF024398DO
MSDS
Size Pls inquire
Source in vitro E.coli expression system
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Product Details

Target Names
TRDN
Uniprot No.
Alternative Names
TRDN; Triadin
Species
Canis lupus familiaris (Dog) (Canis familiaris)
Expression Region
1-701
Target Protein Sequence
MTEITAEGNASTTTTVIDSKNGSVPKSPGKVLKRTVTEDIVTTFSSPAAWLLVIALIITWSAVAVVMFDLVDYKNFSASSLSKIGSDPLKLVHDAVEETTDWVYGFFSLLSDIISSDGDEDDDDGDEDTDKGEIEEPPLKQKEIHKEKAEKEEKPERKILAKVAHREKEKVKEKEKSEKKATHKEKIEKKEKPETKTMAKEERKAKTEEKIKKEVKGGKQEKVKPTAAKVKEVQKTPPKAKEKEGKETAAVAKHEQKDQYAFCRYMIDMFVHGDLRPGQSPALPPPLPTVQASRPTPASPTLEGKEEEEKKKAEKKVTSETKKKEKEDVKKKSDKDTAIDVEKKEPGKAPETKQGTIKVVAQAAAKKDEKKEDSKKTKTPVEEHPKGKKQEKKEKYVEPAKSSKKEHSAPSEKQVKAKTERAKEETSAASTKKAVPGKKEEKTTKTVEQEIRKEKSGKTSTASKDKEPEIKKDEKMPKADKEVKPKPPQSQVKKEEKSESQVKKEAKPEQDIAKPEKTVSHGKPEEKVVKQVKATEKAAIEKTVKPKPAKKAEHQEKESPTIKTDKPKPTSKETPEVTESGKKKIEKSEKESKEKAEMKHLKEEKVSTRKESLQSHNVTKAEKPARVSREDLEDVSASKKAKEEAEDVSSTKRQKSPISFFQCVYLDGYNGYGFQFPVTPAYRPGESSGQPSSPGQKQQGQ
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Contributes to the regulation of lumenal Ca2+ release via the sarcoplasmic reticulum calcium release channels RYR1 and RYR2, a key step in triggering skeletal and heart muscle contraction. Required for normal organization of the triad junction, where T-tubules and the sarcoplasmic reticulum terminal cisternae are in close contact. Required for normal skeletal muscle strength. Plays a role in excitation-contraction coupling in the heart and in regulating the rate of heart beats.
Gene References into Functions
  1. TRD enhances cardiac excitation-contraction coupling by directly stimulating the RyR2. Interaction of TRD with RyR2 may involve amino acids 200 to 224 in C-terminal domain of TRD. PMID: 15731460
  2. proteasome inhibition led to an accumulation of two new modified forms of triadin-1 that were seen with triadin-1 only when it is not glycosylated on Asn(75). PMID: 18025088
Subcellular Location
Sarcoplasmic reticulum membrane; Single-pass type II membrane protein.
Tissue Specificity
Detected in heart (at protein level). Skeletal and cardiac muscle.
Database Links

KEGG: cfa:403776

UniGene: Cfa.2651

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