Recombinant Halobacterium salinarum Cobalamin import system permease protein BtuC (btuC)

Code CSB-CF532244HTL(A4)
MSDS
Size $1620
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  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-CF532244HTL(A4) could indicate that this peptide derived from E.coli-expressed Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1) btuC.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-CF532244HTL(A4) could indicate that this peptide derived from E.coli-expressed Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1) btuC.
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Product Details

Purity
Greater than 85% as determined by SDS-PAGE.
Target Names
btuC
Uniprot No.
Research Area
Others
Alternative Names
btuC; OE_2952F; Cobalamin import system permease protein BtuC
Species
Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1)
Source
in vitro E.coli expression system
Expression Region
1-369aa
Target Protein Sequence
MREASARAVAWSAAAGVLLVAVLLVSATIGPEPITLRTVAMAALTELAVPVGASVTMHTHAVPVVSGGLPWPALTIAYAAPLQFGVPETAQVIVGTIRLPRIVLGATVGASLAISGAVLQGFFRNPMADPSIVGVSSGAAVGAVAAITLPSVVVIGVQPAAFAGALIAAFTVYAIATKNGHTPTATLLLSGVAVQTLLGAVTSFLVVNSGREIRPAMYWLMGTLHGSRWHDVEAALPVVVVGSAVLLAYAREMNVLLAGEEDAHTLGVDVDRTKRLLLAVASVVTAAAVSFAGAIGFVGLIVPHAVRLVVGPDHRVLLPVSALTGGAFLVAADTVARATATEPPVGIITALIGAPFFLYLLRDREVRAL
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
40.3 kDa
Protein Length
Full Length
Tag Info
N-terminal 10xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

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Target Background

Function
Required for corrinoid utilization. Probably part of the ABC transporter complex BtuCDF involved in cobalamin (vitamin B12) import. Probably involved in the translocation of the substrate across the membrane.
Subcellular Location
Cell membrane; Multi-pass membrane protein.
Protein Families
Binding-protein-dependent transport system permease family, FecCD subfamily
Database Links

KEGG: hsl:OE_2952F

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