Recombinant Human Bax inhibitor 1 (TMBIM6)

Code CSB-CF023632HU
MSDS
Size Pls inquire
Source in vitro E.coli expression system
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Product Details

Target Names
TMBIM6
Uniprot No.
Alternative Names
TMBIM6; BI1; TEGT; Bax inhibitor 1; BI-1; Testis-enhanced gene transcript protein; Transmembrane BAX inhibitor motif-containing protein 6
Species
Homo sapiens (Human)
Expression Region
1-237
Target Protein Sequence
MNIFDRKINFDALLKFSHITPSTQQHLKKVYASFALCMFVAAAGAYVHMVTHFIQAGLLS ALGSLILMIWLMATPHSHETEQKRLGLLAGFAFLTGVGLGPALEFCIAVNPSILPTAFMG TAMIFTCFTLSALYARRRSYLFLGGILMSALSLLLLSSLGNVFFGSIWLFQANLYVGLVV MCGFVLFDTQLIIEKAEHGDQDYIWHCIDLFLDFITVFRKLMMILAMNEKDKKKEKK
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Suppressor of apoptosis. Modulates unfolded protein response signaling. Modulates ER calcium homeostasis by acting as a calcium-leak channel. Negatively regulates autophagy and autophagosome formation, especially during periods of nutrient deprivation, and reduces cell survival during starvation.
Gene References into Functions
  1. BI-1 is overexpressed and promotes the progression and metastasis of non-small cell lung cancer PMID: 25973025
  2. These results suggest that the TMBIM family has comparable functions in the maintenance of intracellular Ca(2) homeostasis in a wide variety of tissues PMID: 25764978
  3. Bax inhibitor-1 is likely a pH-sensitive calcium leak channel, not a H+/Ca2+ exchanger. PMID: 25227609
  4. BI-1 enhances glucose metabolism, leading to high activation of the sodium/hydrogen exchange for intracellular pH homeostasis. PMID: 24314142
  5. Results highlight the regulatory role of BI-1 in idiopathic pulmonary fibrosis. PMID: 24625972
  6. The effect of Bax Inhibitor-1(TMBIM6) as a regulatorof endoplasmic reticulum Ca2+ homeostasis is regulated by intracellular pH by removing negative charges. PMID: 23867001
  7. Constructed lentivirus expression vector for transfecting BI-1. PMID: 24344041
  8. Data indicate that GAAP and BI-1 have a six membrane-spanning topology with cytosolic N and C termini and a C-terminal reentrant loop. PMID: 22418439
  9. The results reveal BI-1 as a novel autophagy regulator that bridges Ca(2) signaling between ER and mitochondria, reducing cellular oxygen consumption and contributing to cellular resilience in the face of metabolic stress. PMID: 22588718
  10. The C terminus of Bax inhibitor-1 forms a Ca2+-permeable channel pore. PMID: 22128171
  11. Lentivirus-mediated RNA interference targeting Bax inhibitor-1 suppresses ex vivo cell proliferation and in vivo tumor growth of nasopharyngeal carcinoma. PMID: 21545297
  12. highly maintained lysosomal activity may be one of the mechanisms by which BI-1 exerts its regulatory effects on the ER stress response and cell death. PMID: 21586565
  13. expression of BAX inhibitor-1 is associated with acidic pH-induced calcium release, cell death, and pro-inflammatory cytokine release in human osteoblasts PMID: 21601004
  14. Brain tissue from BI-1 transgenic mice shows reduced levels of apoptotic cells and reduced induction of markers of endoplasmic reticulum stress after brain injury. PMID: 21075086
  15. These findings suggest that BI-1 can lead cancer invasion and metastasis by inducing extracellular environment acidic. PMID: 21537843
  16. Studies indicate that manipulation of BI-1 has the potential for significant therapeutic benefit in a wide range of diseases. PMID: 21297665
  17. the pathway by which estrogen induces apoptosis is possibly through an up-regulation of Klf10 that decreases BI-1 and finally increases the concentration of cytoplasmic calcium PMID: 21262377
  18. post-translational regulation of the BI-1 protein by E3 ligase BAR contributes to the dynamic control of IRE1 signaling during endoplasmic reticulum stress PMID: 21068390
  19. BI-1 expression is down-regulated as liver damage progresses. PMID: 20359348
  20. Data show that BI-1 increased actin polymerization and cell adhesion through Ca2+ regulation and actin interaction. PMID: 20123969
  21. down-regulation of BI-1 lead to the activation of the associated cell death pathways PMID: 19579059
  22. Results indicate that the human Bax inhibitor-1 gene may serve as a prostate cancer expression marker based on its overexpression in prostate carcinoma and prostate cancer cell lines. PMID: 12875974
  23. BI-1 gene expression is conserved evolutionarily and may act as a key regulator of the apoptotic pathway in bronchiolalveolar carcinoma PMID: 16353209
  24. BI-1 can inhibit the endoplasmic reticulum stress proteins as well as the accumulation of Rreactive oxygen species, thereby protecting the cells. PMID: 17526500
  25. BI-1 and Bcl-X(L) operate downstream of or parallel to Bax/Bak PMID: 18299329
  26. BI-1 increases Ca(2+) leak rates from the ER through a mechanism that is dependent on pH and on the carboxyl-terminal cytosolic region of the BI-1 protein. PMID: 18378668
  27. The tmbim6 may participate in cell death regulation by interacting with proteins of Bcl-2 family, promoting tumor metastasis, which is deduced from the evolutionary conservation of the membrane protein family containing multiple membrane spanning segments PMID: 18440869
  28. Results report the simultaneous refolding and reconstitution of the recombinant Bax inhibitor-1 (BI-1) from inclusion bodies expressed in Escherichia coli. PMID: 19284981
  29. These results suggest that reconstituted Bax inhibitor-1 has a Ca(2+)/H(+) antiporter-like activity. PMID: 19290886
  30. Bax inhibitor 1 regulates ER-stress-induced ROS accumulation through the regulation of cytochrome P450 2E1. PMID: 19339548
  31. Bax inhibitor-1 overexpression in 3T3-L1 preadipocytes inhibits calcium mobilizing agent-induced suppression of adipogenesis. PMID: 19433315

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Subcellular Location
Endoplasmic reticulum membrane; Multi-pass membrane protein.
Protein Families
BI1 family
Tissue Specificity
Highly abundant in testis.
Database Links

HGNC: 11723

OMIM: 600748

KEGG: hsa:7009

STRING: 9606.ENSP00000389277

UniGene: Hs.743965

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