Purity
Greater than 90% as determined by SDS-PAGE.
Alternative Names
WNT2B; WNT13; Protein Wnt-2b; Protein Wnt-13
Species
Homo sapiens (Human)
Source
in vitro E.coli expression system
Expression Region
1-391aa
Target Protein Sequence
MLRPGGAEEAAQLPLRRASAPVPVPSPAAPDGSRASARLGLACLLLLLLLTLPARVDTSWWYIGALGARVICDNIPGLVSRQRQLCQRYPDIMRSVGEGAREWIRECQHQFRHHRWNCTTLDRDHTVFGRVMLRSSREAAFVYAISSAGVVHAITRACSQGELSVCSCDPYTRGRHHDQRGDFDWGGCSDNIHYGVRFAKAFVDAKEKRLKDARALMNLHNNRCGRTAVRRFLKLECKCHGVSGSCTLRTCWRALSDFRRTGDYLRRRYDGAVQVMATQDGANFTAARQGYRRATRTDLVYFDNSPDYCVLDKAAGSLGTAGRVCSKTSKGTDGCEIMCCGRGYDTTRVTRVTQCECKFHWCCAVRCKECRNTVDVHTCKAPKKAEWLDQT
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length
Tag Info
N-terminal 6xHis-SUMO-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The expression region of this recombinant Human WNT2B covers amino acids 1-391. The expected molecular weight for the WNT2B protein is calculated to be 59.8 kDa. Expression of this WNT2B protein is conducted in in vitro e.coli expression system. The N-terminal 6xHis-SUMO tag was fused into the coding gene segment of WNT2B, making it easier to detect and purify the WNT2B recombinant protein in the later stages of expression and purification.
The protein Wnt-2b (WNT2B) is a highly researched protein with primary areas of investigation spanning developmental biology, cell signaling, and tumorigenesis. In developmental biology, WNT2B, as a member of the Wnt family, plays a crucial role in embryonic development and organ formation, particularly in the development of the nervous system and muscle tissues. In cell signaling, WNT2B regulates various cellular functions, including proliferation, differentiation, and migration, through the Wnt signaling pathway. In the context of tumor development, the aberrant expression of WNT2B is associated with the occurrence and progression of multiple cancers, making it an important target in cancer biology research.
