Recombinant Human RING finger protein 121 (RNF121)

Code CSB-CF019824HU
MSDS
Size Pls inquire
Source in vitro E.coli expression system
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Product Details

Target Names
RNF121
Uniprot No.
Alternative Names
RNF121; RING finger protein 121
Species
Homo sapiens (Human)
Expression Region
1-327
Target Protein Sequence
MAAVVEVEVGGGAAGERELDEVDMSDLSPEEQWRVEHARMHAKHRGHEAMHAEMVLILIA TLVVAQLLLVQWKQRHPRSYNMVTLFQMWVVPLYFTVKLHWWRFLVIWILFSAVTAFVTF RATRKPLVQTTPRLVYKWFLLIYKISYATGIVGYMAVMFTLFGLNLLFKIKPEDAMDFGI SLLFYGLYYGVLERDFAEMCADYMASTIGFYSESGMPTKHLSDSVCAVCGQQIFVDVSEE GIIENTYRLSCNHVFHEFCIRGWCIVGKKQTCPYCKEKVDLKRMFSNPWERPHVMYGQLL DWLRYLVAWQPVIIGVVQGINYILGLE
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Gene References into Functions
  1. Study found that RNF121 was less expressed in tumor tissues than adjacent normal tissues of renal cell carcinoma (RCC) patients. Overexpression of RNF121 inhibited the growth of human RCC cells in vivo. Moreover, RNF121 impeded the proliferation, migration and invasion of human RCC cells in vitro. In addition, RNF121 was found to activate NF-kappaB signaling pathway via promoting IkappaBalpha degradation in RCC cells. PMID: 30149063
  2. Over-expression of RNF121 promoted ubiquitination of VEGFR-2, inhibited its maturation . RNF121 knockdown in primary endothelial cells reduced VEGFR-2 ubiquitination and increased its cell surface level. PMID: 26602861
  3. These results unveil an unexpected role of Golgi Apparatus and reveal RNF121 as a new player involved in the signaling leading to NF-kappaB activation. PMID: 25388546
  4. This is the first study to demonstrate that RNF121 is a novel regulator of apoptosis and provides a new potential target for cancer therapy PMID: 24928685
Subcellular Location
Membrane; Multi-pass membrane protein.
Protein Families
RNF121 family
Database Links

HGNC: 21070

KEGG: hsa:55298

STRING: 9606.ENSP00000354571

UniGene: Hs.368554

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