Recombinant Human herpesvirus 1 Protein UL20 (UL20)

Code CSB-CF319870HWY
MSDS
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Source in vitro E.coli expression system
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Product Details

Target Names
UL20
Uniprot No.
Alternative Names
UL20; Protein UL20
Species
Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
Expression Region
1-222
Target Protein Sequence
MTMRDDLPLVDRDLVDEAAFGGEEGELPLEEQFSLSSYGTSDFFVSSAYSRLPPHTQPVF SKRVILFLWSFLVLKPLEMVAAGMYYGLTGRVVAPACILAAIVGYYVTWAVRALLLYVNI KRDRLPLSAPVFWGMSVFLGGTALCALFAAAHETFSPDGLFHFIATNQMLPPTDPLRTRA LGIACAAGASMWVAAADSFAASANFFLARFWTRAILNAPVAF
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Plays an essential role in egress of virus particles from the nucleus, cytoplasmic envelopment and virus-induced cell fusion. Forms a functional protein complex with gK and this interaction is absolutely essential for their coordinate intracellular transport, gK glycosylation, expression on host cell surface, and function. Together, they modulate gB-mediated virus-induced cell fusion and virion egress and therefore actively participate in these processes.
Gene References into Functions
  1. Absence of zinc finger protein GODZ affects primary and latent infection, as well as reactivation, in ocularly infected mice. The reduced virus infectivity is due to the absence of the GODZ interaction with HSV-1 UL20. These results strongly suggest that binding of UL20 to GODZ promotes virus infectivity in vitro and viral pathogenesis in vivo. PMID: 29187538
  2. binding of UL20 to GODZ in the Golgi apparatus regulates trafficking of UL20 and its subsequent effects on glycoprotein K localization and virus replication. PMID: 28724772
  3. These data show that efficient endocytosis of gD and gH/gL and their incorporation into mature virions relies upon the presence of the herpes simplex virus type 1 envelope proteins gM and the gK/pUL20 complex. PMID: 25746217
  4. UL20 protein and the amino terminus of gK physically interact with gB. PMID: 20573833
  5. gK and UL20p directly interact and that this interaction facilitates their TGN localization, an important prerequisite for cytoplasmic virion envelopment and egress. PMID: 15542677
  6. role in membrane fusion and virion de-envelopment PMID: 15596825
  7. UL20/gK are required and function prior to UL11 protein in virion cytoplasmic envelopment PMID: 17215291
  8. These results show that UL20p functions in cytoplasmic envelopment are separable from UL20 functions in UL20p intracellular transport, cell surface expression and virus-induced cell fusion. PMID: 17996071
  9. The amino terminus of UL20p interacted with the intracellular gK domain III amd this fusion protien serves important functions in the coordinate transport of UL20p and gK to the trans-Golgi network. PMID: 18434401

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Subcellular Location
Virion. Host Golgi apparatus membrane; Multi-pass membrane protein. Host nucleus membrane; Multi-pass membrane protein.
Protein Families
Alphaherpesvirinae UL20 family
Database Links

KEGG: vg:2703371

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