Recombinant Lactococcus lactis subsp. lactis Multidrug resistance ABC transporter ATP-binding and permease protein (lmrA)

Code CSB-CF880302LNG
MSDS
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Source in vitro E.coli expression system
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Product Details

Target Names
lmrA
Uniprot No.
Alternative Names
lmrA; LL0711; L116532; Multidrug resistance ABC transporter ATP-binding and permease protein
Species
Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Expression Region
1-590
Target Protein Sequence
MERGPQMANRIEGKAVDKTSIKHFIKLIRAAKPRYLFFIIGILAGIVGTLIQLQVPKMVQ PLVNSFGHGVNGGKVALVIALYIGSAAVSAIAAIVLGIFGESVVKNLRTRVWDKMIHLPV KYFDEVKTGEMSSRLANDTTQVKNLIANSIPQAFTSILLLVGSIVFMLQMQWRLTLAMII AVPVVMLIMFPIMTFGQKIGRTRQDSLANFQGIASESLSEIRLVKSSNAEKQASKKAEND VNALYKIGVKEAIFDGLMSPVMMLSMMLMIFGLLAYGIYLISTGVMSLGTLLGMMMYLMN LIGAVPTVATFFTELAKASGSTGRLTELLDEEQEVLHQGESLDLEGKTLSARHVDFAYDD SEQILRDISFEAQPNSIIAFAGPSGGGKSTIFSLLERFYQPTAGEITIDGQPIDNISLEN WRSQIGFVSQDSAIMAGTIRENLTYGLEGDYTDEDLWQVLDLAFARSFVENMPDQLNTEV GERGVKISGGQRQRLAIARAFLRNPKILMLDEATASLDSESESMVQKALDSLMKGRTTLV IAHRLSTIVDADKIYFIEKGQITGSGKHNELVATHPLYAKYVSEQLTVGQ
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Efflux transporter for a variety of amphiphilic cationic compounds, including antibiotics.
Gene References into Functions
  1. analysis of the effect of detergents on the activity of the ABC transporter LmrA PMID: 21651889
  2. a carboxylate has a critical role in proton conduction by the ATP-binding cassette multidrug transporter LmrA PMID: 16040836
  3. LmrA-mediated Hoechst 33342 transport in membrane vesicles is influenced by the transmembrane pH gradient due to a pH-dependent partitioning of Hoechst 33342 into the membrane. PMID: 16363806
  4. Remodelling of LmrA using the atomic coordinates of the MsbA homologue Sav1866 from Staphylococcus aureus is reported. PMID: 17624317
  5. The membrane domain of LmrA mediates an ethidium/proton co-transport reaction, suggesting a functional similarity in the mechanism of secondary-active and ABC transporters. PMID: 14685244
  6. LmrA mediates the reverse uptake of ethidium in ATP-depleted cells in the presence of an inwardly directed ethidium concentration gradient and LmrA-mediated uptake of ethidium is coupled to the catalytic cycle in the direction of ATP synthesis PMID: 14660649

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Subcellular Location
Cell membrane; Multi-pass membrane protein.
Protein Families
ABC transporter superfamily, Multidrug exporter LmrA (TC 3.A.1.117.1) family
Database Links

KEGG: lla:L116532

STRING: 272623.L116532

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