Recombinant Medicago truncatula Calcium and calcium/calmodulin-dependent serine/threonine-protein kinase DMI-3 (DMI3)

Code CSB-CF744189MQP
MSDS
Size Pls inquire
Source in vitro E.coli expression system
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Product Details

Target Names
DMI3
Uniprot No.
Alternative Names
CCAMK; DMI3; MTR_8g043970; Calcium and calcium/calmodulin-dependent serine/threonine-protein kinase DMI-3; CCaMK DMI3; MtCCaMK; Protein DOES NOT MAKE INFECTIONS 3
Species
Medicago truncatula (Barrel medic) (Medicago tribuloides)
Expression Region
1-523
Target Protein Sequence
MGYGTRKLSDEYEVSEILGRGGFSVVRKGTKKSSIEEEKSQSQVAIKTLRRLGASNNPSG LPRKKDIGEKSTIGFPTMRQVSVSDTLLTNEILVMRRIVENVSPHPNVIDLYDVYEDTNG VHLVLELCSGGELFDRIVAQDKYSETEAATVVHQIASGLEAVHRANIVHRDLKPENCLFL DVRKDSPLKIMDFGLSSVEEFTDPVVGLFGSIDYVSPEALSQGKITTKSDMWSLGVILYI LLSGYPPFIAQNNRQKQQMIMNGNFSFYEKTWKGISQPAKNLISSLLTVDPSKRPSALEL LSDPWVKGEKAKDVQMDPEIVSRLQSFNARRKLRAAAIASVWSSTIFLRTKKLKSLVGSY DLKEEEIENLRMHFKKICADRDNATLSEFEEVLKAMNMLSLIPFASRIFDLFDNNRDGTV DMREILCGFSSLKNSKGEDALRLCFQMYDTDRSGCISKEEVASMLRALPYDCLPTDITEP GKLDEIFDLMDANNDGKVTFDEFKAAMQRDSSLQDVVLSSIRP
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
During nodulation, plays a central role in bacterial infection and contributes to nodule organogenesis. Protein kinase that recognizes the calcium spiking induced by Nod factors and translates this signal to components controlling nodulation and mycorrhizal infection responses. May phosphorylate the NSP1 protein. Required in epidermal and cortical cells to promote infection thread (IT) formation in root hairs.
Gene References into Functions
  1. Phosphorylation at S344 completely turns off the root nodule symbiosis-supporting function of CCaMK, even in the T271A gain-of-function background. PMID: 23869591
  2. The visinin-like domain binds three calcium ions, leading to a conformational change involving the exposure of hydrophobic surfaces and a change in tertiary but not net secondary or quaternary structure. PMID: 22889004
Subcellular Location
Nucleus.
Protein Families
Protein kinase superfamily, CAMK Ser/Thr protein kinase family, CaMK subfamily
Tissue Specificity
Highly expressed in roots. Expressed in root hairs and nodules. Expressed at low levels in flowers. Not detected in leaves or stems.
Database Links

KEGG: mtr:MTR_8g043970

UniGene: Mtr.6758

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