Recombinant Mouse 3-oxo-5-alpha-steroid 4-dehydrogenase 1 (Srd5a1)

Code CSB-CF721219MO
MSDS
Size Pls inquire
Source in vitro E.coli expression system
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Product Details

Target Names
Srd5a1
Uniprot No.
Alternative Names
Srd5a1; 3-oxo-5-alpha-steroid 4-dehydrogenase 1; SR type 1; Steroid 5-alpha-reductase 1; S5AR 1
Species
Mus musculus (Mouse)
Expression Region
1-255
Target Protein Sequence
MELDELCLLDALVYLEGFLAFVAFVGLQMVGSSYGRYSSQWSGRRVPARPAWFLQELPSM AWPLYECIRPAAARLGNLPNRVLLAMFLIHYVQRTLVFPVLIRGGKPTLLFTFVLAFLFC TLNGYLQSRYLSQFAVYAEDWVTHPCFLTGFALWLVGMVINIHSDHILRNLRKPGETGYK IPRGGLFEYVSSANYFGELVEWCGFALASWSLQGVVFALFTLCALFTRARQHHQWYLEKF EDYPKTRKILIPFLL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Converts testosterone into 5-alpha-dihydrotestosterone and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology.
Gene References into Functions
  1. Prostate 5aR1 levels were positively correlated with adjusted prostate most severe lesion scores. Downregulation of androgen receptor and 5alpha-reductase 2, along with upregulation of 5a-reductase 1 in tumors may promote prostatic intraepithelial neoplasia and prostate cancer development in TRAMP mice PMID: 28493878
  2. Exaggerated predisposition to metabolic disorders in female mice, compared with that seen in male mice, after disruption of 5alphaR1 suggests phenotypic changes may be underpinned by altered metabolism of glucocorticoids rather than androgens. PMID: 27647861
  3. the Srd5a1 gene modulates ethanol consumption in a sex-dependent manner that is also contingent upon ethanol access condition and concentration PMID: 25416204
  4. deletion of the Srd5a1 gene significantly decreased ethanol's anxiolytic effect in female mice, and chronic ethanol withdrawal severity was lower in female versus male mice PMID: 25355320
  5. transcription of srd5a1 in brain is regulated by environmental factor-induced cytosine methylation in the promoter region. PMID: 26314512
  6. The effects of deficiency or inhibition of S5AR1 on the risk of liver disease and insulin resistance in male rats and mice are reported. PMID: 25239636
  7. The deletion of Type One 5alpha-reductase among female mice attenuates reproductive responding during the estrous cycle. PMID: 24650589
  8. These studies showed that 5alphaR1 deletion accelerates the development of hepatic steatosis but may protect against the development of nonalcoholic fatty liver disease-related hepatocellular neoplasia. PMID: 24080367
  9. Thus, 5alpha-reductase may be required for the estrous cycle variations in affective behavior and 3alpha,5alpha-THP levels of female mice. PMID: 21946309
  10. results suggest that local steroid 5 alpha-reductase 1 messenger RNA expression in Withdrawal Seizure-Prone mouse brain may not change in parallel with local allopregnanolone content or withdrawal severity PMID: 21917407
  11. The aim of the present study was to investigate the role of 5alpha-reductase type 1 for bone mass using Srd5a1-/- mice. PMID: 21731732
  12. This study evaluates 5alpha-R type I and 3alpha-HSD mRNA expression level in mouse brain by using in situ hybridization. PMID: 16984997
  13. social isolation-induced increase of the contextual fear-conditioning response is associated with a marked (50%) down-regulation of 5-RI mRNA expression in the hippocampus, amygdala, and FC. PMID: 18391192
  14. Data suggests that in male mice after social isolation, expression of Srd5a1 mRNA is specifically down-regulated in glutamatergic pyramidal neurons. In socially isolated mice, this down-regulation may account for the appearance of behavioral disorders. PMID: 18003893

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Subcellular Location
Microsome membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein.
Protein Families
Steroid 5-alpha reductase family
Database Links
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